Detailed information of TA system TA235540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4196884..4197544
• Replicon: chromosome
• Accession: NZ_CP092039
• Organism: Pectobacterium brasiliense strain 130

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: MBA20_RS18335
• Protein ID: WP_048256537.1
• Coordinates: 4196884..4197258 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: MBA20_RS18340
• Protein ID: WP_005971623.1
• Coordinates: 4197278..4197544 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA20_RS18310 (MBA20_18310)	4192682..4194010	+	1329	WP_039282120.1	MFS transporter	-
MBA20_RS18315 (MBA20_18315)	4194215..4194601	+	387	WP_048256533.1	acyl-CoA thioesterase	-
MBA20_RS18320 (MBA20_18320)	4194944..4195462	+	519	WP_119870590.1	flavodoxin FldB	-
MBA20_RS18325 (MBA20_18325)	4195491..4195814	+	324	WP_119870589.1	hypothetical protein	-
MBA20_RS18330 (MBA20_18330)	4195880..4196815	-	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
MBA20_RS18335 (MBA20_18335)	4196884..4197258	-	375	WP_048256537.1	protein YgfX	Toxin
MBA20_RS18340 (MBA20_18340)	4197278..4197544	-	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
MBA20_RS18345 (MBA20_18345)	4197780..4198781	+	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
MBA20_RS18350 (MBA20_18350)	4198836..4199483	-	648	WP_010279833.1	hemolysin III family protein	-
MBA20_RS18355 (MBA20_18355)	4199914..4200522	+	609	WP_010279828.1	HD domain-containing protein	-
MBA20_RS18360 (MBA20_18360)	4200577..4201581	+	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14668.36 Da
• Isoelectric Point: 10.5560

>T235540 WP_048256537.1 NZ_CP092039:c4197258-4196884 [Pectobacterium brasiliense]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 375 bp

>T235540 NZ_LR134302:c418211-417765 [Achromobacter spanius]
ATCGCCGTGATAAGTGACGCGCGCGTTTTTCTTGATAGCAACATCCTGATCTATCTGCTGAGCGCCGATGCACGCCGGGC
CGACATTGCTGAAGTCCTGCTCAAAGGCAAGCCATTCATCAGCGTCCAGGTGCTGAATGAGGTAACGAATGTCTGCGTGC
GCAAGCTGAAAAGACAGTGGGAAGAGACTAACGAGTTTCTGGCAATGGTCAGGATGTTCTGTCGTGTTGTGCCACTGACT
GTTGACGTCCACGACAAGGCGCGTCAATTGGCTGAACGCTATCAAATGTCGTTCTATGACGCTTGCATCGCGGCATCGGC
GTTGGCCGTCGACTGCCAGATTCTCTACACGGAAGACATGCAGGCCGATATGTCGATTGATCAGCGGCTCACGCTTATCA
ACCCGTTTACGCAACAGCGTTCGCGCTTGGCTGAGCCGGGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT235540 WP_005971623.1 NZ_CP092039:c4197544-4197278 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT235540 NZ_LR134302:c418431-418195 [Achromobacter spanius]
ATGCGAGTCGCCAAGTGGGGAAATAGTCTGGCCGTGCGTCTTCCCGCCAGCGTGGTTGATGCGTTGAATCTGCAAGAGGG
CGACGATATCGAAATCCACATTCAGGGTGATCGTCATTTCGAAGTGCGCAAGACGGCCGGCGAGGAAGGGGTATTGGCGA
AGCTGCGTCAATTCCGCGGAATGTTGCCGGCAGACTTTGTTTTTAACCGTGACGAGGCGAATCGCCGTGATAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6