Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2056573..2057163 Replicon chromosome
Accession NC_011993
Organism Escherichia coli LF82

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag LF82_RS10410 Protein ID WP_000373724.1
Coordinates 2056573..2056905 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LF82_RS10415 Protein ID WP_000288812.1
Coordinates 2056906..2057163 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LF82_RS10390 2052036..2053298 + 1263 Protein_1977 integrase arm-type DNA-binding domain-containing protein -
LF82_RS10395 2053437..2053895 - 459 WP_000526101.1 IS200/IS605 family transposase -
LF82_RS10400 2054847..2055725 - 879 WP_000511773.1 integrase domain-containing protein -
LF82_RS25290 2055863..2056189 + 327 Protein_1980 hypothetical protein -
LF82_RS10405 2056170..2056526 + 357 WP_001147230.1 hypothetical protein -
LF82_RS10410 2056573..2056905 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LF82_RS10415 2056906..2057163 - 258 WP_000288812.1 antitoxin Antitoxin
LF82_RS25295 2057261..2057452 + 192 WP_077561361.1 hypothetical protein -
LF82_RS10420 2057511..2057717 - 207 WP_000983730.1 helix-turn-helix domain-containing protein -
LF82_RS10425 2057714..2058181 - 468 WP_000201267.1 hypothetical protein -
LF82_RS10430 2058414..2059550 - 1137 WP_000420971.1 ISAs1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2053437..2059550 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T23554 WP_000373724.1 NC_011993:c2056905-2056573 [Escherichia coli LF82]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T23554 NC_011993:c2056905-2056573 [Escherichia coli LF82]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT23554 WP_000288812.1 NC_011993:c2057163-2056906 [Escherichia coli LF82]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT23554 NC_011993:c2057163-2056906 [Escherichia coli LF82]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References