Detailed information of TA system TA235537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2991732..2992285
• Replicon: chromosome
• Accession: NZ_CP092039
• Organism: Pectobacterium brasiliense strain 130

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MBA20_RS13280
• Protein ID: WP_119870392.1
• Coordinates: 2991732..2992046 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3S1FM98
• Locus tag: MBA20_RS13285
• Protein ID: WP_029729596.1
• Coordinates: 2992049..2992285 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA20_RS13260 (MBA20_13260)	2986791..2988653	+	1863	WP_119870396.1	type IV secretory system conjugative DNA transfer family protein	-
MBA20_RS13265 (MBA20_13265)	2988662..2989408	+	747	WP_119870395.1	MobC family replication-relaxation protein	-
MBA20_RS13270 (MBA20_13270)	2989605..2990147	+	543	WP_119870394.1	DUF3916 domain-containing protein	-
MBA20_RS13275 (MBA20_13275)	2990310..2991308	-	999	WP_119870393.1	hypothetical protein	-
MBA20_RS13280 (MBA20_13280)	2991732..2992046	-	315	WP_119870392.1	CcdB family protein	Toxin
MBA20_RS13285 (MBA20_13285)	2992049..2992285	-	237	WP_029729596.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MBA20_RS13290 (MBA20_13290)	2992605..2993549	-	945	WP_119870391.1	zincin-like metallopeptidase domain-containing protein	-
MBA20_RS13295 (MBA20_13295)	2994314..2996623	+	2310	WP_119870906.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2966675..3006868	40193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11569.51 Da
• Isoelectric Point: 7.9859

>T235537 WP_119870392.1 NZ_CP092039:c2992046-2991732 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIMIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKASIDFLIDGF

Nucleotide

• Length: 315 bp

>T235537 NZ_LR134300:c5799466-5799185 [Pseudomonas fluorescens]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8927.96 Da
• Isoelectric Point: 6.4612

>AT235537 WP_029729596.1 NZ_CP092039:c2992285-2992049 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT235537 NZ_LR134300:c5799690-5799463 [Pseudomonas fluorescens]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
TCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S1FM98