Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 831077..831717 Replicon chromosome
Accession NZ_CP092039
Organism Pectobacterium brasiliense strain 130

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag MBA20_RS03675 Protein ID WP_039278477.1
Coordinates 831301..831717 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag MBA20_RS03670 Protein ID WP_039278475.1
Coordinates 831077..831304 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA20_RS03645 (MBA20_03645) 826374..826853 - 480 WP_014916572.1 Hcp family type VI secretion system effector -
MBA20_RS03650 (MBA20_03650) 826978..827475 - 498 WP_039278469.1 M48 family metallopeptidase -
MBA20_RS03655 (MBA20_03655) 827559..828752 - 1194 WP_119872012.1 amino acid aminotransferase -
MBA20_RS03660 (MBA20_03660) 828816..829430 - 615 WP_119872013.1 YitT family protein -
MBA20_RS03665 (MBA20_03665) 829681..830160 - 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
MBA20_RS03670 (MBA20_03670) 831077..831304 + 228 WP_039278475.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MBA20_RS03675 (MBA20_03675) 831301..831717 + 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
MBA20_RS03680 (MBA20_03680) 831856..832494 - 639 WP_039517591.1 hypothetical protein -
MBA20_RS03685 (MBA20_03685) 832487..833569 - 1083 WP_119872014.1 alanine racemase -
MBA20_RS03690 (MBA20_03690) 833611..835017 - 1407 WP_010279223.1 replicative DNA helicase -
MBA20_RS03695 (MBA20_03695) 835146..836132 + 987 WP_040033638.1 quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T235535 WP_039278477.1 NZ_CP092039:831301-831717 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T235535 NZ_LR134300:c3983559-3983212 [Pseudomonas fluorescens]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGCACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT235535 WP_039278475.1 NZ_CP092039:831077-831304 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT235535 NZ_LR134300:c3983820-3983569 [Pseudomonas fluorescens]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References