Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 183729..184346 Replicon chromosome
Accession NZ_CP092039
Organism Pectobacterium brasiliense strain 130

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag MBA20_RS00810 Protein ID WP_010281507.1
Coordinates 184167..184346 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag MBA20_RS00805 Protein ID WP_014916995.1
Coordinates 183729..184139 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA20_RS00790 (MBA20_00790) 179593..180636 - 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -
MBA20_RS00795 (MBA20_00795) 180945..183143 - 2199 WP_104209601.1 primosomal protein N' -
MBA20_RS00800 (MBA20_00800) 183417..183632 + 216 WP_010281510.1 50S ribosomal protein L31 -
MBA20_RS00805 (MBA20_00805) 183729..184139 - 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MBA20_RS00810 (MBA20_00810) 184167..184346 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
MBA20_RS00815 (MBA20_00815) 184430..185440 - 1011 WP_119870817.1 iron ABC transporter permease -
MBA20_RS00820 (MBA20_00820) 185437..186399 - 963 WP_014916993.1 ABC transporter substrate-binding protein -
MBA20_RS00825 (MBA20_00825) 186409..187206 - 798 WP_119870818.1 ABC transporter ATP-binding protein -
MBA20_RS00830 (MBA20_00830) 187440..187757 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
MBA20_RS00835 (MBA20_00835) 187945..189105 + 1161 WP_039465321.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T235534 WP_010281507.1 NZ_CP092039:c184346-184167 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T235534 NZ_LR134300:c3491616-3491206 [Pseudomonas fluorescens]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT235534 WP_014916995.1 NZ_CP092039:c184139-183729 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT235534 NZ_LR134300:c3491846-3491616 [Pseudomonas fluorescens]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References