Detailed information of TA system TA235533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 159915..160549
• Replicon: chromosome
• Accession: NZ_CP092039
• Organism: Pectobacterium brasiliense strain 130

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MBA20_RS00680
• Protein ID: WP_014917023.1
• Coordinates: 159915..160190 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: MBA20_RS00685
• Protein ID: WP_014917022.1
• Coordinates: 160187..160549 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA20_RS00665 (MBA20_00665)	156935..158305	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
MBA20_RS00670 (MBA20_00670)	158302..159000	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
MBA20_RS00675 (MBA20_00675)	159154..159678	+	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
MBA20_RS00680 (MBA20_00680)	159915..160190	+	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
MBA20_RS00685 (MBA20_00685)	160187..160549	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MBA20_RS00690 (MBA20_00690)	160923..162110	+	1188	WP_080162913.1	sugar transporter	-
MBA20_RS00695 (MBA20_00695)	162183..163085	+	903	WP_119870811.1	CDF family cation-efflux transporter FieF	-
MBA20_RS00700 (MBA20_00700)	163293..164255	+	963	WP_010281567.1	6-phosphofructokinase	-
MBA20_RS00705 (MBA20_00705)	164536..165420	+	885	WP_014917008.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T235533 WP_014917023.1 NZ_CP092039:159915-160190 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T235533 NZ_LR134300:c3424387-3423632 [Pseudomonas fluorescens]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT235533 WP_014917022.1 NZ_CP092039:160187-160549 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT235533 NZ_LR134300:c3424890-3424522 [Pseudomonas fluorescens]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCTTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7