Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 145850..146601 Replicon chromosome
Accession NZ_CP092039
Organism Pectobacterium brasiliense strain 130

Toxin (Protein)


Gene name ataT Uniprot ID A0A433N1V3
Locus tag MBA20_RS00610 Protein ID WP_010281603.1
Coordinates 146119..146601 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A0M2EWB4
Locus tag MBA20_RS00605 Protein ID WP_039317395.1
Coordinates 145850..146128 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA20_RS00575 (MBA20_00575) 141009..141923 - 915 WP_119870802.1 NAD(P)-dependent oxidoreductase -
MBA20_RS00580 (MBA20_00580) 142261..143019 + 759 WP_119870824.1 DNA-binding transcriptional repressor YgbI -
MBA20_RS00585 (MBA20_00585) 143240..143638 + 399 WP_127110965.1 hypothetical protein -
MBA20_RS00590 (MBA20_00590) 143635..144483 - 849 WP_119870804.1 DUF4225 domain-containing protein -
MBA20_RS00595 (MBA20_00595) 144485..144964 - 480 WP_039511462.1 Hcp family type VI secretion system effector -
MBA20_RS00600 (MBA20_00600) 145459..145740 + 282 WP_230857154.1 hypothetical protein -
MBA20_RS00605 (MBA20_00605) 145850..146128 + 279 WP_039317395.1 DUF1778 domain-containing protein Antitoxin
MBA20_RS00610 (MBA20_00610) 146119..146601 + 483 WP_010281603.1 GNAT family N-acetyltransferase Toxin
MBA20_RS00615 (MBA20_00615) 146734..148068 + 1335 WP_119870806.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MBA20_RS00620 (MBA20_00620) 148141..149028 + 888 WP_010281599.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MBA20_RS00625 (MBA20_00625) 149054..149899 + 846 WP_010281598.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MBA20_RS00630 (MBA20_00630) 149925..150998 + 1074 WP_119870807.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-144)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17509.30 Da        Isoelectric Point: 9.0439

>T235532 WP_010281603.1 NZ_CP092039:146119-146601 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Download         Length: 483 bp

>T235532 NZ_LR134300:2498993-2499571 [Pseudomonas fluorescens]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCTGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.12 Da        Isoelectric Point: 4.3611

>AT235532 WP_039317395.1 NZ_CP092039:145850-146128 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Download         Length: 279 bp

>AT235532 NZ_LR134300:2498350-2498904 [Pseudomonas fluorescens]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCATCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAACCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A433N1V3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2EWB4

References