Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4110096..4110691 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag MBA16_RS20230 Protein ID WP_000239577.1
Coordinates 4110341..4110691 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag MBA16_RS20225 Protein ID WP_001223208.1
Coordinates 4110096..4110347 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS20215 (4105761) 4105761..4109540 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
MBA16_RS20220 (4109543) 4109543..4109884 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
MBA16_RS20225 (4110096) 4110096..4110347 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
MBA16_RS20230 (4110341) 4110341..4110691 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
MBA16_RS20235 (4110771) 4110771..4111301 - 531 WP_000055075.1 inorganic diphosphatase -
MBA16_RS20240 (4111611) 4111611..4112567 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
MBA16_RS20245 (4112707) 4112707..4114209 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
MBA16_RS20250 (4114223) 4114223..4115245 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T235528 WP_000239577.1 NZ_CP092037:4110341-4110691 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T235528 NZ_LR134299:4828206-4828484 [Pseudomonas putida]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT235528 WP_001223208.1 NZ_CP092037:4110096-4110347 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT235528 NZ_LR134299:4828493-4828792 [Pseudomonas putida]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References