Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4014233..4014730 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MBA16_RS19735 Protein ID WP_001173343.1
Coordinates 4014557..4014730 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MBA16_RS19730 Protein ID WP_000398619.1
Coordinates 4014233..4014529 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS19710 (4010964) 4010964..4011683 - 720 WP_000611288.1 two-component system response regulator DcuR -
MBA16_RS19715 (4011680) 4011680..4013311 - 1632 WP_001216477.1 sensor histidine kinase -
MBA16_RS19720 (4013492) 4013492..4013722 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MBA16_RS19725 (4013734) 4013734..4014006 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
MBA16_RS19730 (4014233) 4014233..4014529 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MBA16_RS19735 (4014557) 4014557..4014730 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MBA16_RS19740 (4014849) 4014849..4016366 - 1518 WP_001295074.1 lysine--tRNA ligase -
MBA16_RS19745 (4016603) 4016603..4018060 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T235527 WP_001173343.1 NZ_CP092037:4014557-4014730 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T235527 NZ_LR134299:c3825654-3825217 [Pseudomonas putida]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCTGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCTACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCCGGCTGGTCTGCTCAGCCTGAGTTGACCCGAACGCTGGGCAATCGCTTTCTGGATGACTGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACGCCGGACAGCCGCCTGTTCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT235527 WP_000398619.1 NZ_CP092037:4014233-4014529 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT235527 NZ_LR134299:c3826100-3825651 [Pseudomonas putida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGTGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCCGTGTTCGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAACGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References