Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2749502..2750156 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MBA16_RS13645 Protein ID WP_000244777.1
Coordinates 2749502..2749909 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MBA16_RS13650 Protein ID WP_000354046.1
Coordinates 2749890..2750156 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS13625 (2745459) 2745459..2747192 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
MBA16_RS13630 (2747198) 2747198..2747908 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MBA16_RS13635 (2747933) 2747933..2748829 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MBA16_RS13640 (2748941) 2748941..2749462 + 522 WP_001055874.1 flavodoxin FldB -
MBA16_RS13645 (2749502) 2749502..2749909 - 408 WP_000244777.1 protein YgfX Toxin
MBA16_RS13650 (2749890) 2749890..2750156 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MBA16_RS13655 (2750399) 2750399..2751379 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MBA16_RS13660 (2751575) 2751575..2752234 - 660 WP_000250274.1 hemolysin III family protein -
MBA16_RS13665 (2752398) 2752398..2752709 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MBA16_RS13670 (2752754) 2752754..2754187 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
MBA16_RS13675 (2754244) 2754244..2754987 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T235523 WP_000244777.1 NZ_CP092037:c2749909-2749502 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T235523 NZ_LR134299:c1368762-1368448 [Pseudomonas putida]
ATGATCTTCATCGAGACCCCAGTATTCACCTCCGATCTCAAGGAGCACCTGGATGATGAAGAATACCGGGCTTTGCAGGC
CTATCTGGCCGAGCATCCCGAAGCCGGTTCTCTCCTGGAGGAAACCGGCGGCCTCAGAAAGATCCGCTGGGCAGCCAAGG
GCAAGGGCAAGAGTGGGGGTGTACGAGTGATCTACTACCACGTCACTGCAGCCCACCAGATCCGTATGATTCTGATCTAT
CGCAAGGGCATCGTGGATACCTTGACAAGCAGTCAGAAAGCGCAGCTTCGCGCACTAAACAAAGGGTGGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT235523 WP_000354046.1 NZ_CP092037:c2750156-2749890 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT235523 NZ_LR134299:c1368448-1368161 [Pseudomonas putida]
ATGGAAAAGCATCTCTTCGATCGTCTTGTGGAAAGCATGACTCAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCCTC
GCGTGAGTTCCACGTAGACGCACTTCAAGTCAAAAAGATTCGTCAAGCCACTGGCCTTTCCCAGGCTGCGTTTGCCAAAC
GTATCGATGTCGCAGTAGGAACTCTACGGAATTGGGAACAGGGCCGTCGCGAACCTGAAGGACCAGCTCGTGCCTTGCTT
CGAGCGATTCATAACGATCCCGAACATGTACTTGCGGCCTTGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References