Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | rnlAB/RnlA-RnlB |
| Location | 2493710..2495147 | Replicon | chromosome |
| Accession | NZ_CP092037 | ||
| Organism | Escherichia coli strain MG1655-377GD | ||
Toxin (Protein)
| Gene name | rnlA | Uniprot ID | P52129 |
| Locus tag | MBA16_RS12410 | Protein ID | WP_000155570.1 |
| Coordinates | 2493710..2494783 (+) | Length | 358 a.a. |
Antitoxin (Protein)
| Gene name | rnlB | Uniprot ID | P52130 |
| Locus tag | MBA16_RS12415 | Protein ID | WP_000461704.1 |
| Coordinates | 2494776..2495147 (+) | Length | 124 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MBA16_RS12395 (2489143) | 2489143..2491332 | - | 2190 | WP_000136402.1 | DEAD/DEAH box helicase | - |
| MBA16_RS12400 (2491329) | 2491329..2492945 | - | 1617 | WP_000445380.1 | anti-bacteriophage protein AbpA | - |
| MBA16_RS12405 (2493305) | 2493305..2493568 | - | 264 | WP_000502050.1 | DUF1524 domain-containing protein | - |
| MBA16_RS12410 (2493710) | 2493710..2494783 | + | 1074 | WP_000155570.1 | type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA | Toxin |
| MBA16_RS12415 (2494776) | 2494776..2495147 | + | 372 | WP_000461704.1 | type II toxin-antitoxin system antitoxin RnlB | Antitoxin |
| MBA16_RS12420 (2495502) | 2495502..2496365 | + | 864 | WP_000787597.1 | GTPase family protein | - |
| MBA16_RS12425 (2496457) | 2496457..2497278 | + | 822 | WP_000197391.1 | DUF932 domain-containing protein | - |
| MBA16_RS12430 (2497495) | 2497495..2498196 | + | 702 | WP_000189409.1 | WYL domain-containing protein | - |
| MBA16_RS12435 (2498237) | 2498237..2498473 | + | 237 | WP_001144029.1 | protein YpjK | - |
| MBA16_RS12440 (2498473) | 2498473..2498916 | + | 444 | WP_000824222.1 | lipoprotein YfjS | - |
| MBA16_RS12445 (2498940) | 2498940..2499407 | + | 468 | WP_000702495.1 | protein YfjT | - |
| MBA16_RS12450 (2499794) | 2499794..2499946 | - | 153 | Protein_2430 | arsenate reductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 358 a.a. Molecular weight: 40061.13 Da Isoelectric Point: 6.8293
>T235521 WP_000155570.1 NZ_CP092037:2493710-2494783 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
Download Length: 1074 bp
>T235521 NZ_LR134298:549801-550127 [Pasteurella multocida subsp. gallicida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA
Antitoxin
Download Length: 124 a.a. Molecular weight: 13664.69 Da Isoelectric Point: 4.2391
>AT235521 WP_000461704.1 NZ_CP092037:2494776-2495147 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
Download Length: 372 bp
>AT235521 NZ_LR134298:550207-550533 [Pasteurella multocida subsp. gallicida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGCCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGCCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|