235503

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 253792..254471 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag MBA16_RS01220 Protein ID WP_000854672.1
Coordinates 253792..254133 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag MBA16_RS01225 Protein ID WP_000070395.1
Coordinates 254154..254471 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS01195 (249141) 249141..249470 + 330 Protein_231 sigma factor-binding protein Crl -
MBA16_RS01200 (249509) 249509..250564 - 1056 WP_000749863.1 phosphoporin PhoE -
MBA16_RS01205 (250852) 250852..251955 + 1104 WP_001285288.1 glutamate 5-kinase -
MBA16_RS01210 (251967) 251967..253220 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
MBA16_RS01220 (253792) 253792..254133 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
MBA16_RS01225 (254154) 254154..254471 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
MBA16_RS01230 (254498) 254498..254974 - 477 WP_000811693.1 RadC family protein -
MBA16_RS01235 (254990) 254990..255448 - 459 WP_000211838.1 antirestriction protein -
MBA16_RS01240 (255546) 255546..255785 - 240 WP_000194654.1 DUF905 family protein -
MBA16_RS01245 (255862) 255862..256329 - 468 WP_001547765.1 protein YkfB -
MBA16_RS01250 (256352) 256352..256795 - 444 WP_000824223.1 lipoprotein YafY -
MBA16_RS01255 (256795) 256795..256971 - 177 WP_001285112.1 hypothetical protein -
MBA16_RS01260 (257018) 257018..257209 - 192 Protein_243 DeoR family transcriptional regulator -
MBA16_RS01265 (257426) 257426..258247 - 822 WP_000197389.1 DUF932 domain-containing protein -
MBA16_RS01270 (258339) 258339..259202 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T235503 WP_000854672.1 NZ_CP092037:c254133-253792 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T235503 NZ_LR134296:199991-200098 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT235503 WP_000070395.1 NZ_CP092037:c254471-254154 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT235503 NZ_LR134296:c199943-199877 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References