Detailed information of TA system TA235497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2502821..2503386
• Replicon: chromosome
• Accession: NZ_CP092033
• Organism: Lacticaseibacillus rhamnosus strain VHProbi Y39

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: LT346_RS11585
• Protein ID: WP_005692155.1
• Coordinates: 2502821..2503168 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LT346_RS11590
• Protein ID: WP_020752297.1
• Coordinates: 2503168..2503386 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT346_RS11565 (LT346_11565)	2498316..2499557	-	1242	WP_005692160.1	acyltransferase	-
LT346_RS11570 (LT346_11570)	2499554..2500255	-	702	WP_005692159.1	ATP-binding cassette domain-containing protein	-
LT346_RS11575 (LT346_11575)	2500248..2501066	-	819	WP_014571588.1	ABC transporter permease subunit	-
LT346_RS11580 (LT346_11580)	2501307..2501975	-	669	WP_005692157.1	phenylalanine--tRNA ligase beta subunit-related protein	-
LT346_RS11585 (LT346_11585)	2502821..2503168	-	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LT346_RS11590 (LT346_11590)	2503168..2503386	-	219	WP_020752297.1	hypothetical protein	Antitoxin
LT346_RS11595 (LT346_11595)	2503480..2505315	-	1836	WP_015764721.1	ABC transporter ATP-binding protein	-
LT346_RS11600 (LT346_11600)	2505302..2507092	-	1791	WP_015764722.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T235497 WP_005692155.1 NZ_CP092033:c2503168-2502821 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T235497 NZ_LR134295:3473456-3473674 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8117.33 Da
• Isoelectric Point: 5.7995

>AT235497 WP_020752297.1 NZ_CP092033:c2503386-2503168 [Lacticaseibacillus rhamnosus]
MMDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 219 bp

>AT235497 NZ_LR134295:3473056-3473430 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAACTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75