Detailed information of TA system TA235489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 940912..942632
• Replicon: chromosome
• Accession: NZ_CP092014
• Organism: Edwardsiella ictaluri strain 13 TAL-140 K3

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: MAY91_RS04545
• Protein ID: WP_049640352.1
• Coordinates: 941976..942632 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MAY91_RS04540
• Protein ID: WP_049640353.1
• Coordinates: 940912..941979 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY91_RS04525 (MAY91_04515)	936152..936706	+	555	WP_049640356.1	hypothetical protein	-
MAY91_RS04530 (MAY91_04520)	936937..937653	-	717	WP_049640355.1	SprT family zinc-dependent metalloprotease	-
MAY91_RS04535 (MAY91_04525)	937653..940748	-	3096	WP_049640354.1	HsdR family type I site-specific deoxyribonuclease	-
MAY91_RS04540 (MAY91_04530)	940912..941979	-	1068	WP_049640353.1	macro domain-containing protein	Antitoxin
MAY91_RS04545 (MAY91_04535)	941976..942632	-	657	WP_049640352.1	DUF4433 domain-containing protein	Toxin
MAY91_RS04550 (MAY91_04540)	942645..944012	-	1368	WP_241772364.1	restriction endonuclease subunit S	-
MAY91_RS04555 (MAY91_04545)	944006..946444	-	2439	WP_049640351.1	type I restriction-modification system subunit M	-
MAY91_RS04560 (MAY91_04550)	946483..946722	-	240	WP_015872655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	934381..948331	13950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25092.60 Da
• Isoelectric Point: 7.2044

>T235489 WP_049640352.1 NZ_CP092014:c942632-941976 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSQVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRSVAAQNVPFVFTDSHAYYNWANYYTRLDGLNQIDWPILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T235489 NZ_LR134295:856526-856933 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40668.97 Da
• Isoelectric Point: 6.9066

>AT235489 WP_049640353.1 NZ_CP092014:c941979-940912 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTDKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT235489 NZ_LR134295:856279-856545 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA