Detailed information of TA system TA235425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67437..68251
• Replicon: chromosome
• Accession: NZ_CP092004
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N14-1660

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A3G3DUX8
• Locus tag: MA620_RS00350
• Protein ID: WP_000971653.1
• Coordinates: 67437..67964 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: M7S6I2
• Locus tag: MA620_RS00355
• Protein ID: WP_000855694.1
• Coordinates: 67961..68251 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA620_RS00320 (63811)	63811..64209	+	399	Protein_63	cytoplasmic protein	-
MA620_RS00325 (64420)	64420..64638	+	219	Protein_64	hypothetical protein	-
MA620_RS00330 (64795)	64795..65463	+	669	WP_000445914.1	hypothetical protein	-
MA620_RS00335 (65490)	65490..65984	+	495	WP_000424946.1	hypothetical protein	-
MA620_RS00340 (66156)	66156..66812	-	657	WP_023225036.1	protein-serine/threonine phosphatase	-
MA620_RS00345 (67162)	67162..67364	+	203	Protein_68	IS5/IS1182 family transposase	-
MA620_RS00350 (67437)	67437..67964	-	528	WP_000971653.1	GNAT family N-acetyltransferase	Toxin
MA620_RS00355 (67961)	67961..68251	-	291	WP_000855694.1	DUF1778 domain-containing protein	Antitoxin
MA620_RS00360 (68522)	68522..68722	-	201	Protein_71	transposase	-
MA620_RS00365 (68963)	68963..69289	+	327	WP_000393295.1	hypothetical protein	-
MA620_RS00370 (69562)	69562..69909	-	348	WP_001555786.1	DUF1493 family protein	-
MA620_RS00375 (69894)	69894..70343	-	450	WP_023225038.1	hypothetical protein	-
MA620_RS00380 (70775)	70775..71218	-	444	WP_023225039.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
MA620_RS00385 (71676)	71676..72326	+	651	WP_072203970.1	type III secretion system transcriptional activator InvF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	67188..68722	1534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19043.87 Da
• Isoelectric Point: 9.7032

>T235425 WP_000971653.1 NZ_CP092004:c67964-67437 [Salmonella enterica subsp. enterica serovar Kentucky]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGRHDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T235425 NZ_LR134264:979124-979474 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.50 Da
• Isoelectric Point: 6.3133

>AT235425 WP_000855694.1 NZ_CP092004:c68251-67961 [Salmonella enterica subsp. enterica serovar Kentucky]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTQMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT235425 NZ_LR134264:978960-979127 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Toxin

• AlphaFold DB: A0A3G3DUX8

Antitoxin

• AlphaFold DB: A0A3V8SJE7