Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4782275..4782900 Replicon chromosome
Accession NZ_CP091992
Organism Klebsiella pneumoniae strain NB04

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MA881_RS26245 Protein ID WP_002882817.1
Coordinates 4782517..4782900 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MA881_RS26240 Protein ID WP_004150355.1
Coordinates 4782275..4782517 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MA881_RS26215 4778266..4778865 + 600 WP_004151865.1 glucose-1-phosphatase -
MA881_RS26220 4778859..4779719 + 861 WP_002882807.1 virulence factor BrkB family protein -
MA881_RS26225 4779716..4780153 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MA881_RS26230 4780198..4781139 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MA881_RS26235 4781153..4782070 - 918 WP_002882812.1 alpha/beta hydrolase -
MA881_RS26240 4782275..4782517 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MA881_RS26245 4782517..4782900 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MA881_RS26250 4783074..4784003 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MA881_RS26255 4784000..4784635 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MA881_RS26260 4784632..4785534 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T235361 WP_002882817.1 NZ_CP091992:4782517-4782900 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T235361 NZ_LR134240:2704553-2704660 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT235361 WP_004150355.1 NZ_CP091992:4782275-4782517 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT235361 NZ_LR134240:c2704506-2704440 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References