Detailed information of TA system TA023536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 3147717..3148245
• Replicon: chromosome
• Accession: NC_011989
• Organism: Agrobacterium vitis S4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B9JS92
• Locus tag: AVI_RS14925
• Protein ID: WP_015917132.1
• Coordinates: 3147717..3147998 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: B9JS93
• Locus tag: AVI_RS14930
• Protein ID: WP_015917133.1
• Coordinates: 3147985..3148245 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI_RS14910	3145219..3145785	+	567	WP_015917129.1	sarcosine oxidase subunit gamma	-
AVI_RS14915	3146108..3147169	-	1062	WP_015917130.1	SDR family oxidoreductase	-
AVI_RS14920	3147268..3147660	+	393	WP_015917131.1	helix-turn-helix transcriptional regulator	-
AVI_RS14925	3147717..3147998	-	282	WP_015917132.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AVI_RS14930	3147985..3148245	-	261	WP_015917133.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AVI_RS14935	3148402..3148650	+	249	WP_015917134.1	antitoxin	-
AVI_RS14940	3148640..3148930	+	291	WP_015917135.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AVI_RS14945	3149289..3149756	+	468	WP_015917136.1	hypothetical protein	-
AVI_RS14950	3149874..3150146	+	273	WP_015917137.1	BrnT family toxin	-
AVI_RS14955	3150115..3150393	+	279	WP_041697087.1	helix-turn-helix domain-containing protein	-
AVI_RS14960	3150428..3151534	-	1107	WP_015917139.1	TRAP transporter substrate-binding protein	-
AVI_RS14965	3151736..3152500	+	765	WP_015917140.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10859.50 Da
• Isoelectric Point: 8.0739

>T23536 WP_015917132.1 NC_011989:c3147998-3147717 [Agrobacterium vitis S4]
VRKIEKTGQFKRDLKRESKGRYRTILEDELLPVIAALAQDEVLEARYRDHALTGEWKDHRDCHVKPDLVLIYRKPDAELL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T23536 NC_011989:c3147998-3147717 [Agrobacterium vitis S4]
GTGCGAAAAATTGAGAAAACCGGGCAATTTAAGCGTGATTTGAAGCGAGAGTCCAAGGGGCGCTATCGCACAATTCTCGA
AGACGAGTTGCTGCCTGTTATCGCCGCTTTGGCTCAGGATGAGGTTCTGGAAGCCAGGTACCGTGATCACGCCCTAACCG
GTGAGTGGAAAGATCACCGTGATTGCCACGTGAAGCCCGATCTGGTGCTGATTTATCGTAAGCCGGATGCTGAACTTCTC
CAGCTTGTTCGACTTGGGTCGCATTCCGAATTGGGTCTGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9127.68 Da
• Isoelectric Point: 6.3081

>AT23536 WP_015917133.1 NC_011989:c3148245-3147985 [Agrobacterium vitis S4]
MAANQLVQARIDGEIKAEATAVLAAMGLTVSDAVRLLLTKVAQEKALPFEPLIPNETTIAAMREARAGKVETVTLDDLKA
AIRAKN

Nucleotide

• Length: 261 bp

>AT23536 NC_011989:c3148245-3147985 [Agrobacterium vitis S4]
ATGGCCGCAAATCAACTGGTTCAAGCCCGTATTGACGGTGAGATCAAAGCAGAAGCAACGGCCGTTCTCGCGGCCATGGG
GCTGACGGTGTCCGATGCGGTGCGGCTGTTGCTGACCAAGGTTGCGCAGGAAAAGGCGCTGCCGTTCGAACCGTTGATCC
CCAACGAAACCACGATTGCCGCCATGCGAGAGGCTCGCGCGGGCAAGGTGGAGACTGTGACGCTCGATGATCTCAAAGCG
GCGATCCGTGCGAAAAATTGA

Structures

Toxin

• AlphaFold DB: B9JS92

Antitoxin

• AlphaFold DB: B9JS93