Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 61005..61815 Replicon chromosome
Accession NZ_CP091992
Organism Klebsiella pneumoniae strain NB04

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MA881_RS03095 Protein ID WP_002887280.1
Coordinates 61282..61815 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MA881_RS03090 Protein ID WP_002887278.1
Coordinates 61005..61271 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MA881_RS03070 56877..57221 - 345 WP_002887266.1 cation efflux system protein CusF -
MA881_RS03075 57239..58624 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MA881_RS03080 58796..59479 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
MA881_RS03085 59469..60902 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MA881_RS03090 61005..61271 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MA881_RS03095 61282..61815 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MA881_RS03100 61863..62984 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T235347 WP_002887280.1 NZ_CP091992:61282-61815 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T235347 NZ_LR134240:194564-194671 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT235347 WP_002887278.1 NZ_CP091992:61005-61271 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT235347 NZ_LR134240:c194516-194450 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References