Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54867..55603 Replicon plasmid pNB04-2
Accession NZ_CP091987
Organism Klebsiella pneumoniae strain NB04

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MA881_RS02165 Protein ID WP_003026803.1
Coordinates 55121..55603 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MA881_RS02160 Protein ID WP_003026799.1
Coordinates 54867..55133 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MA881_RS02115 50929..51291 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
MA881_RS02120 51341..51691 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
MA881_RS02125 52049..52318 + 270 WP_004152102.1 hypothetical protein -
MA881_RS02130 52306..52881 + 576 WP_004152103.1 hypothetical protein -
MA881_RS02135 52912..53406 + 495 WP_004152104.1 DNA-binding protein -
MA881_RS02140 53450..53818 + 369 WP_004152105.1 hypothetical protein -
MA881_RS02145 53852..54055 + 204 WP_004152106.1 HHA domain-containing protein -
MA881_RS02150 54104..54361 + 258 WP_004152107.1 hypothetical protein -
MA881_RS02155 54437..54691 + 255 WP_004152108.1 hypothetical protein -
MA881_RS02160 54867..55133 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MA881_RS02165 55121..55603 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MA881_RS02170 55804..57207 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
MA881_RS02175 57236..57868 - 633 WP_001567369.1 hypothetical protein -
MA881_RS02180 58098..59444 + 1347 WP_020314316.1 ISNCY family transposase -
MA881_RS28715 59605..59736 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) - 1..171032 171032


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T235346 WP_003026803.1 NZ_CP091987:55121-55603 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T235346 NZ_LR134239:c4068297-4068124 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT235346 WP_003026799.1 NZ_CP091987:54867-55133 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT235346 NZ_LR134239:c4068621-4068325 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References