Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | stbDE/ParE-YafN |
| Location | 273079..273601 | Replicon | plasmid pNB04-NDM-1 |
| Accession | NZ_CP091986 | ||
| Organism | Klebsiella pneumoniae strain NB04 | ||
Toxin (Protein)
| Gene name | stbE | Uniprot ID | A0A7H0EVH6 |
| Locus tag | MA881_RS01510 | Protein ID | WP_014839901.1 |
| Coordinates | 273317..273601 (+) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | stbD | Uniprot ID | A0A7H0EVH5 |
| Locus tag | MA881_RS01505 | Protein ID | WP_014839900.1 |
| Coordinates | 273079..273327 (+) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MA881_RS01480 | 268257..268613 | + | 357 | WP_017901071.1 | hypothetical protein | - |
| MA881_RS01485 | 268987..269385 | + | 399 | WP_014839899.1 | H-NS family nucleoid-associated regulatory protein | - |
| MA881_RS01490 | 269522..270130 | + | 609 | WP_024266306.1 | DUF2913 family protein | - |
| MA881_RS01495 | 270674..271306 | + | 633 | WP_001567369.1 | hypothetical protein | - |
| MA881_RS01500 | 271335..272738 | - | 1404 | WP_001567368.1 | ISNCY-like element ISKpn21 family transposase | - |
| MA881_RS01505 | 273079..273327 | + | 249 | WP_014839900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| MA881_RS01510 | 273317..273601 | + | 285 | WP_014839901.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| MA881_RS01515 | 273792..274268 | + | 477 | WP_042934526.1 | hypothetical protein | - |
| MA881_RS01520 | 274274..274951 | + | 678 | WP_042934501.1 | hypothetical protein | - |
| MA881_RS01525 | 274968..275309 | - | 342 | WP_233430673.1 | DUF2807 domain-containing protein | - |
| MA881_RS01530 | 275292..275687 | - | 396 | Protein_306 | cation:proton antiporter | - |
| MA881_RS01535 | 275928..276530 | - | 603 | WP_014839902.1 | HAD domain-containing protein | - |
| MA881_RS01540 | 276527..277048 | - | 522 | WP_048336588.1 | hypothetical protein | - |
| MA881_RS01545 | 277129..278073 | - | 945 | WP_014839903.1 | DsbA family protein | - |
| MA881_RS01550 | 278136..278345 | - | 210 | WP_125316032.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mph(E) / msr(E) / armA / sul1 / qacE / aadA5 / dfrA1 / blaNDM-1 / fosA3 / blaSHV-12 / qnrB4 / blaDHA-1 / qnrB52 / mph(A) | htpB | 1..355227 | 355227 | |
| - | flank | IS/Tn | - | - | 271335..272738 | 1403 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10866.46 Da Isoelectric Point: 10.5831
>T235345 WP_014839901.1 NZ_CP091986:273317-273601 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN
Download Length: 285 bp
>T235345 NZ_LR134239:c3895640-3895266 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9071.29 Da Isoelectric Point: 3.8832
>AT235345 WP_014839900.1 NZ_CP091986:273079-273327 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL
Download Length: 249 bp
>AT235345 NZ_LR134239:c3896098-3895730 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7H0EVH6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7H0EVH5 |