Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 246063..246733 Replicon plasmid pJNQH373-1
Accession NZ_CP091979
Organism Klebsiella pneumoniae isolate JNQH373

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag L6V94_RS27120 Protein ID WP_004213072.1
Coordinates 246063..246506 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag L6V94_RS27125 Protein ID WP_004213073.1
Coordinates 246503..246733 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V94_RS27085 (L6V94_27085) 241475..241750 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
L6V94_RS27090 (L6V94_27090) 241813..242304 + 492 WP_011251327.1 DM13 domain-containing protein -
L6V94_RS27095 (L6V94_27095) 242353..243273 + 921 WP_004213934.1 DUF1471 domain-containing protein -
L6V94_RS27100 (L6V94_27100) 243364..243767 + 404 Protein_252 GAF domain-containing protein -
L6V94_RS27105 (L6V94_27105) 244285..244919 - 635 Protein_253 mucoid phenotype regulator RmpA2 -
L6V94_RS27110 (L6V94_27110) 245336..245640 + 305 Protein_254 transposase -
L6V94_RS27115 (L6V94_27115) 245663..245914 - 252 WP_186987481.1 hypothetical protein -
L6V94_RS27120 (L6V94_27120) 246063..246506 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
L6V94_RS27125 (L6V94_27125) 246503..246733 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6V94_RS27130 (L6V94_27130) 247341..248474 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
L6V94_RS27135 (L6V94_27135) 248490..248783 + 294 WP_004213076.1 hypothetical protein -
L6V94_RS27140 (L6V94_27140) 248773..248979 - 207 WP_004213077.1 hypothetical protein -
L6V94_RS27145 (L6V94_27145) 249331..249621 + 291 WP_004213078.1 hypothetical protein -
L6V94_RS27150 (L6V94_27150) 249611..250510 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroE / iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..279569 279569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T235341 WP_004213072.1 NZ_CP091979:c246506-246063 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T235341 NZ_LR134239:2513985-2514092 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT235341 WP_004213073.1 NZ_CP091979:c246733-246503 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT235341 NZ_LR134239:c2513938-2513872 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References