Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 192213..193123 Replicon plasmid pJNQH373-1
Accession NZ_CP091979
Organism Klebsiella pneumoniae isolate JNQH373

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag L6V94_RS26815 Protein ID WP_004026354.1
Coordinates 192213..192683 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag L6V94_RS26820 Protein ID WP_004026357.1
Coordinates 192680..193123 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V94_RS26790 (L6V94_26790) 187625..188023 - 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -
L6V94_RS26795 (L6V94_26795) 188360..188854 - 495 WP_004212794.1 thermonuclease family protein -
L6V94_RS26800 (L6V94_26800) 189032..189298 + 267 WP_223175074.1 DUF1173 family protein -
L6V94_RS26805 (L6V94_26805) 189331..189981 + 651 WP_068893702.1 DUF1173 family protein -
L6V94_RS26815 (L6V94_26815) 192213..192683 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
L6V94_RS26820 (L6V94_26820) 192680..193123 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
L6V94_RS26825 (L6V94_26825) 193224..193727 - 504 Protein_197 DUF4113 domain-containing protein -
L6V94_RS26830 (L6V94_26830) 193825..194046 - 222 WP_004213643.1 DUF2188 domain-containing protein -
L6V94_RS26835 (L6V94_26835) 194227..194346 - 120 Protein_199 Fe3+-citrate ABC transporter substrate-binding protein -
L6V94_RS26840 (L6V94_26840) 194421..196547 - 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
L6V94_RS26845 (L6V94_26845) 196634..197587 - 954 Protein_201 fec operon regulator FecR -
L6V94_RS26850 (L6V94_26850) 197584..198105 - 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroE / iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..279569 279569
- flank IS/Tn - - 191367..191870 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T235340 WP_004026354.1 NZ_CP091979:c192683-192213 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T235340 NZ_LR134239:2513450-2513557 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT235340 WP_004026357.1 NZ_CP091979:c193123-192680 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT235340 NZ_LR134239:c2513403-2513337 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References