Detailed information of TA system TA023534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2904493..2905081
• Replicon: chromosome
• Accession: NC_011989
• Organism: Agrobacterium vitis S4

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AVI_RS13835
• Protein ID: WP_041697009.1
• Coordinates: 2904493..2904810 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: B9JRA3
• Locus tag: AVI_RS13840
• Protein ID: WP_015916927.1
• Coordinates: 2904824..2905081 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI_RS13815	2901073..2902011	+	939	WP_015916923.1	response regulator	-
AVI_RS13820	2902039..2902674	+	636	WP_041697007.1	HAD-IA family hydrolase	-
AVI_RS13825	2902709..2903242	+	534	WP_015916924.1	AAA family ATPase	-
AVI_RS13830	2903337..2903822	-	486	WP_015916925.1	hypothetical protein	-
AVI_RS13835	2904493..2904810	-	318	WP_041697009.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI_RS13840	2904824..2905081	-	258	WP_015916927.1	antitoxin	Antitoxin
AVI_RS13845	2905175..2906155	-	981	WP_015916928.1	hypothetical protein	-
AVI_RS13850	2906285..2906893	+	609	WP_049777232.1	hypothetical protein	-
AVI_RS13855	2907113..2907373	-	261	WP_015916929.1	cell division topological specificity factor MinE	-
AVI_RS13860	2907370..2908185	-	816	WP_015916930.1	septum site-determining protein MinD	-
AVI_RS13865	2908318..2909118	-	801	WP_015916931.1	septum site-determining protein MinC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11119.87 Da
• Isoelectric Point: 10.4538

>T23534 WP_041697009.1 NC_011989:c2904810-2904493 [Agrobacterium vitis S4]
IWLVSLDLTSGHEQQGTRPVLIISPGAFNRLTKTPVVLPITSGGNFARTAGFAVSLSGVGLRTTGAVRCDQPRALDLAAR
RGKKLESVPSEIVDEVLARLATILE

Nucleotide

• Length: 318 bp

>T23534 NC_011989:c2904810-2904493 [Agrobacterium vitis S4]
ATCTGGTTGGTGTCGCTTGATCTCACATCCGGGCATGAACAACAGGGAACACGTCCGGTGCTGATTATCTCGCCCGGCGC
GTTTAATCGATTGACCAAAACACCTGTGGTTCTGCCTATCACCAGCGGCGGCAATTTTGCGAGGACGGCTGGTTTTGCTG
TGTCGTTGAGCGGGGTTGGTTTACGCACCACGGGCGCCGTGCGCTGTGATCAGCCGCGTGCGCTTGATTTGGCCGCGCGA
AGGGGCAAAAAGCTGGAATCGGTGCCAAGCGAGATTGTCGATGAGGTGTTGGCACGGCTGGCAACGATTCTGGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8942.18 Da
• Isoelectric Point: 4.3287

>AT23534 WP_015916927.1 NC_011989:c2905081-2904824 [Agrobacterium vitis S4]
MHTANLRKVGGSVMVAVPPALLDVLHLSAGATVSMAVDNGRLVIEPQARPRYTLEELLSQCDGSAEVSADDREWLDGGPV
GGELM

Nucleotide

• Length: 258 bp

>AT23534 NC_011989:c2905081-2904824 [Agrobacterium vitis S4]
ATGCATACAGCCAATCTGCGCAAAGTTGGTGGTTCAGTGATGGTGGCTGTTCCGCCTGCTCTGCTGGATGTTTTGCATCT
TAGCGCGGGTGCAACGGTTTCCATGGCGGTGGATAATGGTCGTCTGGTGATCGAGCCGCAGGCGCGGCCTCGTTATACGC
TGGAGGAATTGCTGTCTCAATGCGACGGGTCGGCGGAAGTCTCTGCGGATGATCGGGAGTGGCTTGATGGCGGGCCTGTC
GGTGGAGAGTTGATGTAA

Structures

Antitoxin

• AlphaFold DB: B9JRA3