Detailed information of TA system TA023533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2758851..2759395
• Replicon: chromosome
• Accession: NC_011989
• Organism: Agrobacterium vitis S4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B9JZS9
• Locus tag: AVI_RS13145
• Protein ID: WP_015916808.1
• Coordinates: 2759072..2759395 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B9JZS8
• Locus tag: AVI_RS13140
• Protein ID: WP_015916807.1
• Coordinates: 2758851..2759075 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI_RS13120	2754948..2755766	+	819	WP_015916803.1	ABC transporter ATP-binding protein	-
AVI_RS13125	2755780..2756667	+	888	WP_015916804.1	ABC transporter permease	-
AVI_RS13130	2756922..2758151	-	1230	WP_015916805.1	argininosuccinate synthase	-
AVI_RS13135	2758278..2758745	+	468	WP_015916806.1	Lrp/AsnC family transcriptional regulator	-
AVI_RS13140	2758851..2759075	+	225	WP_015916807.1	antitoxin MazE family protein	Antitoxin
AVI_RS13145	2759072..2759395	+	324	WP_015916808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI_RS13150	2759432..2760838	-	1407	WP_041698153.1	sensor histidine kinase	-
AVI_RS13155	2760869..2761534	-	666	WP_015916810.1	response regulator	-
AVI_RS13160	2761634..2762380	-	747	WP_015916811.1	metallophosphoesterase family protein	-
AVI_RS13165	2762384..2762740	-	357	WP_015916812.1	hydroxyisourate hydrolase	-
AVI_RS13170	2762737..2763246	-	510	WP_015916813.1	ureidoglycolate lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (6-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11530.56 Da
• Isoelectric Point: 10.7588

>T23533 WP_015916808.1 NC_011989:2759072-2759395 [Agrobacterium vitis S4]
VKRGDLVKVAVPGDFGKPRPALIIQSDFFSETGTATVLLISSTLVDAPLLRVMVQPSPANGLKKPSQIMVDKAMSIRRER
LGPAFGRLDDDAMLSVTRSLVVFFGFG

Nucleotide

• Length: 324 bp

>T23533 NC_011989:2759072-2759395 [Agrobacterium vitis S4]
GTGAAGCGGGGCGATCTTGTAAAAGTCGCCGTGCCCGGCGACTTTGGTAAGCCGCGCCCTGCCCTCATCATTCAATCAGA
TTTTTTCTCTGAAACCGGCACCGCAACCGTGTTGCTGATCTCCTCTACCCTTGTCGATGCGCCGCTGCTGCGGGTGATGG
TGCAACCGTCGCCTGCCAATGGCTTGAAAAAACCATCACAGATCATGGTGGACAAAGCGATGTCGATCAGGCGAGAGCGG
CTTGGCCCCGCCTTCGGTCGGTTGGATGATGACGCCATGCTGTCCGTGACCCGTTCGCTGGTGGTGTTTTTCGGCTTTGG
CTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8315.39 Da
• Isoelectric Point: 5.0039

>AT23533 WP_015916807.1 NC_011989:2758851-2759075 [Agrobacterium vitis S4]
MPTPVNHRVQKRRDALRAAGLRPVQIWVPDTRRPGFIEECHRQSMAVAAADGTDGDVLAFMDDALIDLNNEYPE

Nucleotide

• Length: 225 bp

>AT23533 NC_011989:2758851-2759075 [Agrobacterium vitis S4]
TTGCCGACACCCGTCAATCACCGTGTACAAAAACGGCGCGATGCCTTGCGTGCTGCGGGATTGCGACCTGTGCAAATCTG
GGTGCCGGATACGCGTCGGCCTGGTTTTATCGAGGAATGCCATCGTCAATCCATGGCGGTTGCGGCTGCCGATGGGACTG
ATGGTGATGTTCTGGCTTTCATGGATGATGCTCTGATCGATCTCAATAACGAGTATCCCGAGTGA

Structures

Toxin

• AlphaFold DB: B9JZS9

Antitoxin

• AlphaFold DB: B9JZS8