Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1306736..1307361 Replicon chromosome
Accession NZ_CP091978
Organism Klebsiella pneumoniae isolate JNQH373

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag L6V94_RS06420 Protein ID WP_012737091.1
Coordinates 1306978..1307361 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6V94_RS06415 Protein ID WP_004150355.1
Coordinates 1306736..1306978 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V94_RS06390 (1302727) 1302727..1303326 + 600 WP_004151865.1 glucose-1-phosphatase -
L6V94_RS06395 (1303320) 1303320..1304180 + 861 WP_004146232.1 virulence factor BrkB family protein -
L6V94_RS06400 (1304177) 1304177..1304614 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6V94_RS06405 (1304659) 1304659..1305600 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L6V94_RS06410 (1305614) 1305614..1306531 - 918 WP_012737090.1 alpha/beta hydrolase -
L6V94_RS06415 (1306736) 1306736..1306978 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6V94_RS06420 (1306978) 1306978..1307361 + 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
L6V94_RS06425 (1307535) 1307535..1308464 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6V94_RS06430 (1308461) 1308461..1309096 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6V94_RS06435 (1309093) 1309093..1309995 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T235326 WP_012737091.1 NZ_CP091978:1306978-1307361 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T235326 NZ_LR134237:4630477-4630854 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT235326 WP_004150355.1 NZ_CP091978:1306736-1306978 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT235326 NZ_LR134237:4630019-4630387 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGATCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References