Detailed information of TA system TA235318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3977246..3978009
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q5P631
• Locus tag: L8M16_RS19000
• Protein ID: WP_011236954.1
• Coordinates: 3977515..3978009 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: L8M16_RS18995
• Protein ID: WP_011236953.1
• Coordinates: 3977246..3977518 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS18970	3972817..3973893	-	1077	WP_011236948.1	tellurite resistance/C4-dicarboxylate transporter family protein	-
L8M16_RS18975	3973890..3975401	-	1512	WP_011236949.1	PHB depolymerase family esterase	-
L8M16_RS18980	3975568..3976098	-	531	WP_251199511.1	hypothetical protein	-
L8M16_RS18985	3976379..3976795	+	417	WP_011236951.1	pilin	-
L8M16_RS18990	3976988..3977185	+	198	WP_241762814.1	hypothetical protein	-
L8M16_RS18995	3977246..3977518	+	273	WP_011236953.1	DUF1778 domain-containing protein	Antitoxin
L8M16_RS19000	3977515..3978009	+	495	WP_011236954.1	GNAT family N-acetyltransferase	Toxin
L8M16_RS19005	3978209..3978523	+	315	WP_011236955.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
L8M16_RS19010	3978526..3978810	+	285	WP_011236956.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L8M16_RS19015	3978893..3979144	+	252	WP_248280493.1	type II toxin-antitoxin system VapB family antitoxin	-
L8M16_RS19020	3979141..3979524	+	384	WP_011236958.1	twitching motility protein PilT	-
L8M16_RS19025	3979521..3980051	-	531	WP_168953773.1	GNAT family N-acetyltransferase	-
L8M16_RS19030	3980048..3980329	-	282	WP_168953774.1	DUF1778 domain-containing protein	-
L8M16_RS19035	3980453..3981058	-	606	WP_041645864.1	EAL domain-containing protein	-
L8M16_RS19040	3981506..3981784	+	279	WP_041645866.1	DUF1778 domain-containing protein	-
L8M16_RS19045	3981781..3982380	+	600	WP_248280492.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17970.63 Da
• Isoelectric Point: 7.5570

>T235318 WP_011236954.1 NZ_CP091977:3977515-3978009 [Aromatoleum aromaticum]
MSLQLSAPQPLAATHLLDEFASGETCLDEWLKRRALSNQLSGASRTFVVTDQEQHVFGYYAMAAGAVSHQMATGGVRRNM
PDPIPVMVLARLAIDDRVQGIKLGAALLQDAVNRAVAVSQNAGVRALLVHALHEHAKQFYEHYGFQESPQHPMTLMLRLN
TVKA

Nucleotide

• Length: 495 bp

>T235318 NZ_LR134237:2669585-2669863 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9838.30 Da
• Isoelectric Point: 5.0008

>AT235318 WP_011236953.1 NZ_CP091977:3977246-3977518 [Aromatoleum aromaticum]
MRDAAINLRALPEQRDLIDHAASLLGKNRSDFMLEAACDRAQAVVLDQVFFGLDADKFKQFTAMLDAPPGPNPGLERLMA
VQAPWRTGAA

Nucleotide

• Length: 273 bp

>AT235318 NZ_LR134237:2669863-2670147 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: Q5P631