Detailed information of TA system TA235316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 3195119..3195786
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L8M16_RS15275
• Protein ID: WP_197531869.1
• Coordinates: 3195355..3195786 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q5P894
• Locus tag: L8M16_RS15270
• Protein ID: WP_011236200.1
• Coordinates: 3195119..3195349 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS15260	3190972..3192873	+	1902	WP_168954131.1	DNA mismatch repair endonuclease MutL	-
L8M16_RS15265	3192974..3194950	+	1977	WP_011236199.1	DNA topoisomerase IV subunit B	-
L8M16_RS15270	3195119..3195349	+	231	WP_011236200.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L8M16_RS15275	3195355..3195786	+	432	WP_197531869.1	type II toxin-antitoxin system VapC family toxin	Toxin
L8M16_RS15280	3195783..3198143	+	2361	WP_011236202.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15970.25 Da
• Isoelectric Point: 6.3260

>T235316 WP_197531869.1 NZ_CP091977:3195355-3195786 [Aromatoleum aromaticum]
MLDTNICIYLINHRPAHVRAHFDGHPIGDIGVSVITAAELAYGVTKSASPRNRAALETFLLPLEVAPFDDRAIWRYAELR
TQLMRRGTPIGALDTQIAAHALALDCTLVTNNTREFERVDGLRLENWVDQRLMESPAAYFTAP

Nucleotide

• Length: 432 bp

>T235316 NZ_LR134237:c1998140-1997766 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8487.69 Da
• Isoelectric Point: 5.0931

>AT235316 WP_011236200.1 NZ_CP091977:3195119-3195349 [Aromatoleum aromaticum]
MDTAKLFTNGRSQAVRLPKEFRFEGDEVFIKRVGDAVVLLPRHGWGTLFGALEAFEPGFTIERGAPEQAEREALLP

Nucleotide

• Length: 231 bp

>AT235316 NZ_LR134237:c1998597-1998229 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: Q5P894