Detailed information of TA system TA235315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2877563..2878204
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L8M16_RS13740
• Protein ID: WP_011239860.1
• Coordinates: 2877794..2878204 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: Q5NXJ8
• Locus tag: L8M16_RS13735
• Protein ID: WP_011239859.1
• Coordinates: 2877563..2877793 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS13715	2872733..2873677	+	945	WP_011239855.1	flocculation-associated PEP-CTERM protein PepA	-
L8M16_RS13720	2873760..2875286	+	1527	WP_041646646.1	hypothetical protein	-
L8M16_RS13725	2875348..2876421	-	1074	WP_011239857.1	nitroreductase family protein	-
L8M16_RS13730	2876414..2877301	-	888	WP_041646647.1	ThiF family adenylyltransferase	-
L8M16_RS13735	2877563..2877793	+	231	WP_011239859.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L8M16_RS13740	2877794..2878204	+	411	WP_011239860.1	PIN domain-containing protein	Toxin
L8M16_RS13745	2878400..2880292	-	1893	WP_011239861.1	propionate--CoA ligase	-
L8M16_RS13750	2880617..2881615	-	999	WP_011239863.1	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15396.53 Da
• Isoelectric Point: 8.1014

>T235315 WP_011239860.1 NZ_CP091977:2877794-2878204 [Aromatoleum aromaticum]
MSVRYLLDTNICIYIAKHNPPEVRQRFARHAASELAMSVITLGELRFGAEKSQARDKALAAIQRLEATVRIAPLTESVGE
HYGQIRANLQHKGQPIGNNDLWLAAHARAEGWILVTNNEREFVRVEGLQVENWVDR

Nucleotide

• Length: 411 bp

>T235315 NZ_LR134237:1987691-1987945 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAAAACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8676.88 Da
• Isoelectric Point: 4.7863

>AT235315 WP_011239859.1 NZ_CP091977:2877563-2877793 [Aromatoleum aromaticum]
MVYARVFRSGNSQAVRLPKEFRLDTDRVEIFRRGDEIVLRELPGNAAAIFDALIELPVNFMSEGRNDVAPQEREAL

Nucleotide

• Length: 231 bp

>AT235315 NZ_LR134237:1987443-1987694 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: Q5NXJ8