Detailed information of TA system TA235305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParE-CopA/ParE-RHH
• Location: 350705..351276
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q5P3P1
• Locus tag: L8M16_RS01610
• Protein ID: WP_011237766.1
• Coordinates: 350983..351276 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: copA
• Uniprot ID: -
• Locus tag: L8M16_RS01605
• Protein ID: WP_041646138.1
• Coordinates: 350705..350995 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS01585	345747..346238	+	492	WP_011237761.1	MarR family transcriptional regulator	-
L8M16_RS01590	346263..347690	+	1428	WP_011237762.1	efflux transporter outer membrane subunit	-
L8M16_RS01595	347713..349014	+	1302	WP_011237763.1	HlyD family efflux transporter periplasmic adaptor subunit	-
L8M16_RS01600	349032..350576	+	1545	WP_011237764.1	DHA2 family efflux MFS transporter permease subunit	-
L8M16_RS01605	350705..350995	+	291	WP_041646138.1	CopG family ribbon-helix-helix protein	Antitoxin
L8M16_RS01610	350983..351276	+	294	WP_011237766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L8M16_RS01615	351887..353106	-	1220	Protein_317	IS110 family transposase	-
L8M16_RS01620	353459..353638	-	180	WP_049780243.1	hypothetical protein	-
L8M16_RS01625	353861..354136	-	276	WP_041646140.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
L8M16_RS01630	354297..354692	-	396	WP_011237771.1	type II toxin-antitoxin system VapC family toxin	-
L8M16_RS01635	354689..354931	-	243	WP_041646142.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10962.55 Da
• Isoelectric Point: 8.5368

>T235305 WP_011237766.1 NZ_CP091977:350983-351276 [Aromatoleum aromaticum]
MPQVRFAPAALRDLERLRDFLRPKNPAAAKRAAAAIIKAVQVLGQQPHIGRPVADLPEEFREWPIEFGDSGYVARYRYDA
NEDVIILGIRHQKEAGE

Nucleotide

• Length: 294 bp

>T235305 NZ_LR134236:3766812-3767189 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCACACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11219.53 Da
• Isoelectric Point: 5.6919

>AT235305 WP_041646138.1 NZ_CP091977:350705-350995 [Aromatoleum aromaticum]
MPTTAAKTVSVKLDDEMRDRIKHLAETRHRTTHWVMREAIQQYVEREEKREAFRQATLAAWEEYQETGVSVAADEVTAWL
ETWGESNEQAAPKCRK

Nucleotide

• Length: 291 bp

>AT235305 NZ_LR134236:3766402-3766761 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTAATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Toxin

• AlphaFold DB: Q5P3P1