Detailed information of TA system TA235304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 293257..293921
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q5P3U6
• Locus tag: L8M16_RS01335
• Protein ID: WP_011237711.1
• Coordinates: 293502..293921 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L8M16_RS01330
• Protein ID: WP_241762839.1
• Coordinates: 293257..293505 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS01300	288361..288897	+	537	WP_168954281.1	transposase	-
L8M16_RS01305	289280..290404	+	1125	WP_011235911.1	ISAs1-like element ISAzo3 family transposase	-
L8M16_RS01310	290508..291086	+	579	WP_168955051.1	HNH endonuclease	-
L8M16_RS01315	291290..292357	+	1068	WP_168955050.1	IS630 family transposase	-
L8M16_RS01320	292476..292799	-	324	WP_168955049.1	type II toxin-antitoxin system PemK/MazF family toxin	-
L8M16_RS01325	292799..293023	-	225	WP_168955048.1	antitoxin MazE family protein	-
L8M16_RS01330	293257..293505	+	249	WP_241762839.1	Arc family DNA-binding protein	Antitoxin
L8M16_RS01335	293502..293921	+	420	WP_011237711.1	type II toxin-antitoxin system VapC family toxin	Toxin
L8M16_RS01340	294145..294879	-	735	WP_011236010.1	IS21-like element ISAzo4 family helper ATPase IstB	-
L8M16_RS01345	294902..296461	-	1560	WP_041646113.1	IS21-like element ISAzo4 family transposase	-
L8M16_RS01350	296762..297112	+	351	WP_011237713.1	hypothetical protein	-
L8M16_RS01355	297122..297538	+	417	WP_168953785.1	hypothetical protein	-
L8M16_RS01360	297700..298530	-	831	WP_168953786.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	281093..293921	12828
	-	inside	IScluster/Tn	-	-	289280..296461	7181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-120)

Antitoxin

• Arc-like DNA binding domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14858.29 Da
• Isoelectric Point: 6.9803

>T235304 WP_011237711.1 NZ_CP091977:293502-293921 [Aromatoleum aromaticum]
MILLDTNVVSEPLRHAPEVRVIEWIDVQPLETLFLSTITVAELRAGVALLPAGKRRSGLQENLEKRVLPLFAGRVLPFDL
ACTRAYAELMAKARAAGLAIATADGYIAAIAAANGFTVATRYTSPFEAAGAAVINPWQA

Nucleotide

• Length: 420 bp

>T235304 NZ_LR134236:3723517-3723795 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9282.57 Da
• Isoelectric Point: 7.2067

>AT235304 WP_241762839.1 NZ_CP091977:293257-293505 [Aromatoleum aromaticum]
MLTVRNLPEEVHRALRVRAAQHGRSMEAEVREILESVVSPQGRVKLGSLLADLGRQARLSDEEFAVFEQVRDKTPSRPVS
FE

Nucleotide

• Length: 249 bp

>AT235304 NZ_LR134236:3723254-3723514 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: Q5P3U6