Detailed information of TA system TA235302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 287781..288292
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q5P3V9
• Locus tag: L8M16_RS01295
• Protein ID: WP_011237699.1
• Coordinates: 288035..288292 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: L8M16_RS01290
• Protein ID: WP_041646111.1
• Coordinates: 287781..288038 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS01270	283449..284141	+	693	WP_211160012.1	orotate phosphoribosyltransferase	-
L8M16_RS01275	284119..284754	+	636	WP_168954282.1	hypothetical protein	-
L8M16_RS01280	284847..286931	+	2085	WP_011237696.1	molybdopterin oxidoreductase family protein	-
L8M16_RS01285	286924..287706	+	783	WP_011237697.1	NAD-dependent deacylase	-
L8M16_RS01290	287781..288038	+	258	WP_041646111.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
L8M16_RS01295	288035..288292	+	258	WP_011237699.1	Txe/YoeB family addiction module toxin	Toxin
L8M16_RS01300	288361..288897	+	537	WP_168954281.1	transposase	-
L8M16_RS01305	289280..290404	+	1125	WP_011235911.1	ISAs1-like element ISAzo3 family transposase	-
L8M16_RS01310	290508..291086	+	579	WP_168955051.1	HNH endonuclease	-
L8M16_RS01315	291290..292357	+	1068	WP_168955050.1	IS630 family transposase	-
L8M16_RS01320	292476..292799	-	324	WP_168955049.1	type II toxin-antitoxin system PemK/MazF family toxin	-
L8M16_RS01325	292799..293023	-	225	WP_168955048.1	antitoxin MazE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	281093..293921	12828
	-	inside	IScluster/Tn	-	-	289280..296461	7181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10342.78 Da
• Isoelectric Point: 6.7243

>T235302 WP_011237699.1 NZ_CP091977:288035-288292 [Aromatoleum aromaticum]
MRIIFHPQAWDDYLYWQQTDKQILKRVNALIRDIQRDPFDGIGKPEPLKFNLTAYWSRRIDDEHRIVYKVLDDEVIIAQA
RGHYE

Nucleotide

• Length: 258 bp

>T235302 NZ_LR134236:3525090-3525308 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9921.35 Da
• Isoelectric Point: 4.8723

>AT235302 WP_041646111.1 NZ_CP091977:287781-288038 [Aromatoleum aromaticum]
MRAITYTDARNNLASELDRVVNDCDVTIITRQKAEATVLMSMREYESLIETAHLLRNPKNAMRLMKAVDDIENRRNLIER
DLIEE

Nucleotide

• Length: 258 bp

>AT235302 NZ_LR134236:3524690-3525064 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: Q5P3V9