Detailed information of TA system TA023530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 1656524..1657146
• Replicon: chromosome
• Accession: NC_011989
• Organism: Agrobacterium vitis S4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AVI_RS07880
• Protein ID: WP_202909988.1
• Coordinates: 1656790..1657146 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B9JW04
• Locus tag: AVI_RS07875
• Protein ID: WP_015917607.1
• Coordinates: 1656524..1656787 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI_RS07855	1652280..1654301	-	2022	WP_041696520.1	hypothetical protein	-
AVI_RS29530	1654918..1655121	+	204	WP_041696524.1	hypothetical protein	-
AVI_RS07870	1655088..1656101	-	1014	WP_015915854.1	IS1595 family transposase	-
AVI_RS07875	1656524..1656787	+	264	WP_015917607.1	PbsX family transcriptional regulator	Antitoxin
AVI_RS07880	1656790..1657146	+	357	WP_202909988.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI_RS07885	1657659..1658366	-	708	WP_015915855.1	TIGR02281 family clan AA aspartic protease	-
AVI_RS29535	1658370..1658567	-	198	WP_015915856.1	DUF1289 domain-containing protein	-
AVI_RS07890	1658577..1659356	-	780	WP_015915857.1	adenosylcobinamide-GDP ribazoletransferase	-
AVI_RS07895	1659670..1660692	+	1023	WP_015915858.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
AVI_RS07900	1660736..1661125	+	390	WP_041696527.1	diacylglycerol kinase	-
AVI_RS07905	1661157..1661399	+	243	WP_015915860.1	hypothetical protein	-
AVI_RS07910	1661670..1661987	+	318	WP_015915861.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1647482..1657146	9664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12876.79 Da
• Isoelectric Point: 7.8097

>T23530 WP_202909988.1 NC_011989:1656790-1657146 [Agrobacterium vitis S4]
VRSNTPKRGELYLIDLNPVVGNEMRDTHRCVVISPREVNVLGMCLTVVVTTGGQFARRAGLAVNITGHETNGVALCNHVR
SFDILSRVKQKTARHIDTLDSDTVDEIVAKVVGIIEAN

Nucleotide

• Length: 357 bp

>T23530 NC_011989:1656790-1657146 [Agrobacterium vitis S4]
GTGCGAAGTAATACTCCGAAGCGTGGTGAACTGTATCTCATTGATCTTAATCCTGTCGTTGGAAATGAGATGCGAGATAC
TCATCGCTGCGTTGTGATCTCGCCTCGTGAGGTCAATGTCCTCGGAATGTGTTTGACGGTTGTCGTTACGACAGGCGGTC
AGTTTGCGCGAAGAGCGGGTTTAGCCGTAAATATTACTGGCCACGAGACCAACGGCGTCGCTCTTTGCAATCACGTCAGG
AGCTTCGATATCTTATCGCGGGTCAAGCAAAAAACGGCTCGACACATTGACACGCTCGACAGTGACACAGTGGATGAAAT
CGTCGCAAAAGTGGTCGGAATTATCGAGGCCAACTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9081.56 Da
• Isoelectric Point: 8.6164

>AT23530 WP_015917607.1 NC_011989:1656524-1656787 [Agrobacterium vitis S4]
MSNTAKIRRQGGAAVFSIPPALLKMLGADFGTELTLVVNNGALVATPLKPKRKYTLAELLEGSDEMVALSKQAAGWDSGA
PVGNELL

Nucleotide

• Length: 264 bp

>AT23530 NC_011989:1656524-1656787 [Agrobacterium vitis S4]
ATGTCCAACACAGCTAAAATCCGTCGCCAAGGCGGTGCCGCAGTTTTCAGTATTCCCCCCGCTCTCCTGAAAATGCTCGG
AGCTGATTTCGGTACTGAACTCACCCTAGTGGTTAACAACGGCGCACTCGTGGCAACGCCGTTGAAACCAAAGAGGAAAT
ACACCCTTGCCGAGTTGCTTGAGGGATCGGACGAGATGGTGGCTCTTAGCAAACAGGCGGCTGGATGGGACTCGGGTGCA
CCCGTAGGGAATGAGCTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: B9JW04