Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3983421..3984063 Replicon chromosome
Accession NZ_CP091971
Organism Bacillus cereus strain MB1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L7754_RS20305 Protein ID WP_000635963.1
Coordinates 3983713..3984063 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L7754_RS20300 Protein ID WP_000004570.1
Coordinates 3983421..3983708 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7754_RS20275 (L7754_20275) 3978738..3979700 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
L7754_RS20280 (L7754_20280) 3979693..3980265 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
L7754_RS20285 (L7754_20285) 3980359..3980718 + 360 WP_000583416.1 holo-ACP synthase -
L7754_RS20290 (L7754_20290) 3980875..3981825 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
L7754_RS20295 (L7754_20295) 3981943..3983112 + 1170 WP_000390615.1 alanine racemase -
L7754_RS20300 (L7754_20300) 3983421..3983708 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L7754_RS20305 (L7754_20305) 3983713..3984063 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L7754_RS20310 (L7754_20310) 3984131..3986299 + 2169 WP_000426242.1 Tex family protein -
L7754_RS20315 (L7754_20315) 3986357..3986473 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L7754_RS20320 (L7754_20320) 3986669..3987127 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T235298 WP_000635963.1 NZ_CP091971:3983713-3984063 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T235298 NZ_LR134236:2674566-2674673 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT235298 WP_000004570.1 NZ_CP091971:3983421-3983708 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT235298 NZ_LR134236:c2674519-2674453 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References