Detailed information of TA system TA235297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 759276..760254
• Replicon: chromosome
• Accession: NZ_CP091971
• Organism: Bacillus cereus strain MB1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L7754_RS03860
• Protein ID: WP_000624975.1
• Coordinates: 759276..760013 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: L7754_RS03865
• Protein ID: WP_000237818.1
• Coordinates: 760126..760254 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7754_RS03840 (L7754_03840)	755005..755700	+	696	WP_224742171.1	class I SAM-dependent methyltransferase	-
L7754_RS03845 (L7754_03845)	755858..757534	-	1677	WP_139256968.1	alpha-keto acid decarboxylase family protein	-
L7754_RS03850 (L7754_03850)	757651..758133	+	483	WP_072770281.1	MarR family winged helix-turn-helix transcriptional regulator	-
L7754_RS03855 (L7754_03855)	758300..759037	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L7754_RS03860 (L7754_03860)	759276..760013	+	738	WP_000624975.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L7754_RS03865 (L7754_03865)	760126..760254	+	129	WP_000237818.1	hypothetical protein	Antitoxin
L7754_RS03870 (L7754_03870)	760327..760503	+	177	WP_065382523.1	stage II sporulation protein SB	-
L7754_RS03875 (L7754_03875)	760523..760912	-	390	WP_000713862.1	YxeA family protein	-
L7754_RS03880 (L7754_03880)	761124..762593	+	1470	WP_065382524.1	beta-Ala-His dipeptidase	-
L7754_RS03885 (L7754_03885)	762839..763648	+	810	WP_065382626.1	papain-like cysteine protease family protein	-
L7754_RS03890 (L7754_03890)	763674..764276	+	603	WP_065382625.1	hypothetical protein	-
L7754_RS03895 (L7754_03895)	764519..764770	-	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28278.98 Da
• Isoelectric Point: 8.2998

>T235297 WP_000624975.1 NZ_CP091971:759276-760013 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMAIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T235297 NZ_LR134236:c2492044-2491868 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT235297 WP_000237818.1 NZ_CP091971:760126-760254 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT235297 NZ_LR134236:c2491822-2491406 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5