Detailed information of TA system TA235295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 865899..866527
• Replicon: chromosome
• Accession: NZ_CP091967
• Organism: Leptospira noguchii strain barbudensis

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MAL04_RS03505
• Protein ID: WP_017215482.1
• Coordinates: 866126..866527 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MAL04_RS03500
• Protein ID: WP_061248223.1
• Coordinates: 865899..866129 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL04_RS03475 (MAL04_03475)	861505..862644	-	1140	WP_240276754.1	helix-turn-helix domain-containing protein	-
MAL04_RS03480 (MAL04_03480)	862697..862909	-	213	WP_002178978.1	hypothetical protein	-
MAL04_RS03485 (MAL04_03485)	863371..863754	+	384	WP_002178986.1	helix-turn-helix domain-containing protein	-
MAL04_RS03490 (MAL04_03490)	863831..864439	+	609	WP_240276755.1	hypothetical protein	-
MAL04_RS03495 (MAL04_03495)	864470..865375	+	906	WP_240276756.1	histidine kinase N-terminal 7TM domain-containing protein	-
MAL04_RS03500 (MAL04_03500)	865899..866129	+	231	WP_061248223.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAL04_RS03505 (MAL04_03505)	866126..866527	+	402	WP_017215482.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAL04_RS03510 (MAL04_03510)	866727..866918	+	192	WP_235594491.1	nucleotidyltransferase domain-containing protein	-
MAL04_RS03515 (MAL04_03515)	867209..867352	+	144	WP_240278840.1	DUF86 domain-containing protein	-
MAL04_RS03520 (MAL04_03520)	867974..868384	+	411	WP_240276757.1	hypothetical protein	-
MAL04_RS03525 (MAL04_03525)	868440..868727	+	288	WP_240276760.1	hypothetical protein	-
MAL04_RS03530 (MAL04_03530)	868718..869065	+	348	WP_082285430.1	IS66 family insertion sequence element accessory protein TnpB	-
MAL04_RS03535 (MAL04_03535)	869109..870695	+	1587	WP_240276762.1	IS66 family transposase	-
MAL04_RS03540 (MAL04_03540)	870689..871225	+	537	WP_240276764.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15491.14 Da
• Isoelectric Point: 9.2810

>T235295 WP_017215482.1 NZ_CP091967:866126-866527 [Leptospira noguchii]
MTQYLLDTNICIYIINQKPESVYKKFKKIKLENISISSITEFELKYGVQKSLHFEKNLKVLEEFLSYLNILPFVSEDTNK
AAKIRVELEKVGKPIGPFDLLIASQALSNRLTLVTNNEKELNRIKDLKIENWL

Nucleotide

• Length: 402 bp

>T235295 NZ_LR134236:2353822-2354109 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9040.23 Da
• Isoelectric Point: 9.5098

>AT235295 WP_061248223.1 NZ_CP091967:865899-866129 [Leptospira noguchii]
MNKAKIFKNGDSQAIRLPKDYRFKGKEVYIRKDGDNVILTPIDDAVDRFWSTIHNFPDDLIIDRNQPTEYDKRNRI

Nucleotide

• Length: 231 bp

>AT235295 NZ_LR134236:2353583-2353822 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA