Detailed information of TA system TA235294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 18659..19008
• Replicon: plasmid p3_L201601331
• Accession: NZ_CP091966
• Organism: Leptospira noguchii strain 201601331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MAL07_RS19835
• Protein ID: WP_279574428.1
• Coordinates: 18659..18757 (-)
• Length: 33 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MAL07_RS19840
• Protein ID: WP_243825609.1
• Coordinates: 18793..19008 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL07_RS19795 (MAL07_19795)	14639..14904	+	266	Protein_19	IS5/IS1182 family transposase	-
MAL07_RS19800 (MAL07_19800)	15035..15379	+	345	WP_243825608.1	hypothetical protein	-
MAL07_RS19805 (MAL07_19805)	15976..16165	+	190	Protein_21	IS5/IS1182 family transposase	-
MAL07_RS19810 (MAL07_19810)	16427..16829	-	403	Protein_22	putative toxin-antitoxin system toxin component, PIN family	-
MAL07_RS19815 (MAL07_19815)	16839..17072	-	234	Protein_23	ribbon-helix-helix domain-containing protein	-
MAL07_RS19820 (MAL07_19820)	17745..18054	+	310	Protein_24	hypothetical protein	-
MAL07_RS19825 (MAL07_19825)	18020..18393	+	374	Protein_25	hypothetical protein	-
MAL07_RS19830 (MAL07_19830)	18487..18612	-	126	WP_243825619.1	toxin-antitoxin system, toxin component, MazF family protein	-
MAL07_RS19835 (MAL07_19835)	18659..18757	-	99	WP_279574428.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAL07_RS19840 (MAL07_19840)	18793..19008	-	216	WP_243825609.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL07_RS19845 (MAL07_19845)	19522..19917	-	396	WP_243825610.1	hypothetical protein	-
MAL07_RS19850 (MAL07_19850)	20027..20518	-	492	Protein_30	PD-(D/E)XK nuclease family protein	-
MAL07_RS19855 (MAL07_19855)	20795..21215	-	421	Protein_31	XRE family transcriptional regulator	-
MAL07_RS19860 (MAL07_19860)	21284..21502	+	219	WP_243825611.1	hypothetical protein	-
MAL07_RS19865 (MAL07_19865)	21439..21666	+	228	WP_243825620.1	helix-turn-helix transcriptional regulator	-
MAL07_RS19870 (MAL07_19870)	21797..22919	-	1123	Protein_34	restriction endonuclease	-
MAL07_RS19875 (MAL07_19875)	22919..23254	-	336	WP_243825612.1	hypothetical protein	-
MAL07_RS19880 (MAL07_19880)	23534..23779	-	246	WP_243825613.1	DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28484	28484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-30)

Antitoxin

• SpoVT-AbrB domain (2-41)

Sequences

Toxin

Protein

• Length: 33 a.a.
• Molecular weight: 3610.09 Da
• Isoelectric Point: 10.6262

>T235294 WP_279574428.1 NZ_CP091966:c18757-18659 [Leptospira noguchii]
VWLNFTPQAGHEQKGRRPALVLSPKEYNSKTG

Nucleotide

• Length: 99 bp

>T235294 NZ_LR134236:2070122-2070225 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7843.92 Da
• Isoelectric Point: 5.6422

>AT235294 WP_243825609.1 NZ_CP091966:c19008-18793 [Leptospira noguchii]
MGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 216 bp

>AT235294 NZ_LR134236:c2070263-2069992 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT