Detailed information of TA system TA235293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 24315..24886
• Replicon: plasmid p2_L201601331
• Accession: NZ_CP091965
• Organism: Leptospira noguchii strain 201601331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL07_RS19635
• Protein ID: WP_000240418.1
• Coordinates: 24315..24656 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL07_RS19640
• Protein ID: WP_000443825.1
• Coordinates: 24650..24886 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL07_RS19605 (MAL07_19605)	20474..20740	+	267	Protein_14	IS5/IS1182 family transposase	-
MAL07_RS19610 (MAL07_19610)	20886..22005	+	1120	Protein_15	hypothetical protein	-
MAL07_RS19615 (MAL07_19615)	22268..22672	-	405	WP_004420606.1	putative toxin-antitoxin system toxin component, PIN family	-
MAL07_RS19620 (MAL07_19620)	22672..22917	-	246	WP_004420603.1	ribbon-helix-helix domain-containing protein	-
MAL07_RS19625 (MAL07_19625)	23597..23905	+	309	WP_002148401.1	hypothetical protein	-
MAL07_RS19630 (MAL07_19630)	23871..24245	+	375	Protein_19	hypothetical protein	-
MAL07_RS19635 (MAL07_19635)	24315..24656	-	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL07_RS19640 (MAL07_19640)	24650..24886	-	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL07_RS19645 (MAL07_19645)	25200..26411	-	1212	WP_061233587.1	PD-(D/E)XK nuclease family protein	-
MAL07_RS19650 (MAL07_19650)	26652..27074	-	423	WP_061233586.1	hypothetical protein	-
MAL07_RS19655 (MAL07_19655)	27143..27523	+	381	WP_235595273.1	helix-turn-helix transcriptional regulator	-
MAL07_RS19660 (MAL07_19660)	27654..28781	-	1128	WP_061233585.1	hypothetical protein	-
MAL07_RS19665 (MAL07_19665)	28781..29644	-	864	WP_061233588.1	adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38153	38153
	-	flank	IS/Tn	-	-	18968..19816	848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235293 WP_000240418.1 NZ_CP091965:c24656-24315 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235293 NZ_LR134236:c1874150-1873776 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235293 WP_000443825.1 NZ_CP091965:c24886-24650 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235293 NZ_LR134236:c1874608-1874240 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACCCCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33