Detailed information of TA system TA235292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 50518..51089
• Replicon: plasmid p1_L201601331
• Accession: NZ_CP091964
• Organism: Leptospira noguchii strain 201601331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL07_RS19460
• Protein ID: WP_000240418.1
• Coordinates: 50748..51089 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL07_RS19455
• Protein ID: WP_000443825.1
• Coordinates: 50518..50754 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL07_RS19425 (MAL07_19425)	46165..46509	+	345	WP_036026199.1	hypothetical protein	-
MAL07_RS19430 (MAL07_19430)	46496..47038	+	543	WP_061251579.1	hypothetical protein	-
MAL07_RS19435 (MAL07_19435)	47066..47215	+	150	WP_155717419.1	hypothetical protein	-
MAL07_RS19440 (MAL07_19440)	47208..47522	+	315	WP_002149298.1	hypothetical protein	-
MAL07_RS19445 (MAL07_19445)	47957..49246	+	1290	WP_061251578.1	hypothetical protein	-
MAL07_RS19450 (MAL07_19450)	49418..50215	+	798	WP_061251577.1	TIGR02391 family protein	-
MAL07_RS19455 (MAL07_19455)	50518..50754	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL07_RS19460 (MAL07_19460)	50748..51089	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL07_RS19465 (MAL07_19465)	51159..51533	-	375	Protein_37	hypothetical protein	-
MAL07_RS19470 (MAL07_19470)	51499..51807	-	309	WP_002148401.1	hypothetical protein	-
MAL07_RS19475 (MAL07_19475)	51987..52269	-	283	Protein_39	leucine-rich repeat domain-containing protein	-
MAL07_RS20005	52305..52427	+	123	WP_004444280.1	hypothetical protein	-
MAL07_RS19480 (MAL07_19480)	52525..52767	-	243	WP_243825587.1	hypothetical protein	-
MAL07_RS19485 (MAL07_19485)	52764..53657	-	894	WP_243825597.1	Rpn family recombination-promoting nuclease/putative transposase	-
MAL07_RS19490 (MAL07_19490)	54292..55149	-	858	WP_243825588.1	Rpn family recombination-promoting nuclease/putative transposase	-
MAL07_RS19495 (MAL07_19495)	55121..55363	+	243	WP_004426407.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65245	65245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235292 WP_000240418.1 NZ_CP091964:50748-51089 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235292 NZ_LR134236:1859131-1859385 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235292 WP_000443825.1 NZ_CP091964:50518-50754 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235292 NZ_LR134236:1858883-1859134 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33