Detailed information of TA system TA235291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 744763..745339
• Replicon: chromosome
• Accession: NZ_CP091962
• Organism: Leptospira noguchii strain 201601331

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: MAL07_RS02935
• Protein ID: WP_002155521.1
• Coordinates: 744763..745104 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: M6U786
• Locus tag: MAL07_RS02940
• Protein ID: WP_004425715.1
• Coordinates: 745091..745339 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL07_RS02920 (MAL07_02920)	740178..740513	-	336	WP_004426982.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
MAL07_RS02925 (MAL07_02925)	740510..740788	-	279	WP_002155541.1	GNAT family N-acetyltransferase	-
MAL07_RS02930 (MAL07_02930)	741249..743123	+	1875	WP_004443792.1	ATP-dependent DNA helicase RecQ	-
MAL07_RS02935 (MAL07_02935)	744763..745104	-	342	WP_002155521.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAL07_RS02940 (MAL07_02940)	745091..745339	-	249	WP_004425715.1	ribbon-helix-helix domain-containing protein	Antitoxin
MAL07_RS02945 (MAL07_02945)	745607..745879	-	273	WP_004425613.1	BrnA antitoxin family protein	-
MAL07_RS02950 (MAL07_02950)	745866..746165	-	300	WP_004425722.1	BrnT family toxin	-
MAL07_RS02955 (MAL07_02955)	746512..746835	-	324	WP_243825533.1	hypothetical protein	-
MAL07_RS02960 (MAL07_02960)	747117..747698	-	582	WP_232436043.1	hypothetical protein	-
MAL07_RS02965 (MAL07_02965)	748642..749487	-	846	WP_002155522.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12329.22 Da
• Isoelectric Point: 9.8437

>T235291 WP_002155521.1 NZ_CP091962:c745104-744763 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSSISTVVSIAITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Nucleotide

• Length: 342 bp

>T235291 NZ_LR134236:1858883-1859134 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9337.83 Da
• Isoelectric Point: 10.3019

>AT235291 WP_004425715.1 NZ_CP091962:c745339-745091 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSNKSKELKSNIVDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT235291 NZ_LR134236:1859131-1859385 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: M6U786