Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 100003..100574 Replicon plasmid p1_LIP1512017
Accession NZ_CP091959
Organism Leptospira noguchii strain IP1512017

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag MAL03_RS20340 Protein ID WP_000240418.1
Coordinates 100233..100574 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag MAL03_RS20335 Protein ID WP_000443825.1
Coordinates 100003..100239 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAL03_RS20310 (MAL03_20310) 95819..96163 + 345 WP_036026199.1 hypothetical protein -
MAL03_RS20315 (MAL03_20315) 96150..96692 + 543 WP_061251579.1 hypothetical protein -
MAL03_RS20320 (MAL03_20320) 96720..96869 + 150 WP_155717419.1 hypothetical protein -
MAL03_RS20325 (MAL03_20325) 97638..98492 + 855 WP_243816173.1 hypothetical protein -
MAL03_RS20330 (MAL03_20330) 98740..99693 + 954 WP_141599443.1 DUF2971 domain-containing protein -
MAL03_RS20335 (MAL03_20335) 100003..100239 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MAL03_RS20340 (MAL03_20340) 100233..100574 + 342 WP_000240418.1 endoribonuclease MazF Toxin
MAL03_RS20345 (MAL03_20345) 100632..101018 - 387 Protein_91 hypothetical protein -
MAL03_RS20350 (MAL03_20350) 100984..101292 - 309 WP_243816174.1 hypothetical protein -
MAL03_RS20355 (MAL03_20355) 101434..102855 - 1422 WP_243816175.1 leucine-rich repeat domain-containing protein -
MAL03_RS20360 (MAL03_20360) 103037..103206 - 170 Protein_94 AraC family transcriptional regulator -
MAL03_RS20365 (MAL03_20365) 103756..104367 + 612 WP_243816176.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..178101 178101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T235290 WP_000240418.1 NZ_CP091959:100233-100574 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T235290 NZ_LR134236:c1280438-1280058 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT235290 WP_000443825.1 NZ_CP091959:100003-100239 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT235290 NZ_LR134236:c1280659-1280438 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACACTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References