Detailed information of TA system TA235289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 748868..749481
• Replicon: chromosome
• Accession: NZ_CP091957
• Organism: Leptospira noguchii strain IP1512017

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: T0FDD9
• Locus tag: MAL03_RS03135
• Protein ID: WP_002178286.1
• Coordinates: 749221..749481 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MAL03_RS03130
• Protein ID: WP_141598270.1
• Coordinates: 748868..749224 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL03_RS03100 (MAL03_03100)	746247..746588	-	342	WP_002155521.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MAL03_RS03105 (MAL03_03105)	746575..746823	-	249	WP_004425715.1	ribbon-helix-helix domain-containing protein	-
MAL03_RS03110 (MAL03_03110)	747091..747363	-	273	WP_135686010.1	BrnA antitoxin family protein	-
MAL03_RS03115 (MAL03_03115)	747350..747649	-	300	WP_141598267.1	BrnT family toxin	-
MAL03_RS03120 (MAL03_03120)	747974..748364	-	391	Protein_623	type II toxin-antitoxin system VapC family toxin	-
MAL03_RS03125 (MAL03_03125)	748364..748594	-	231	WP_002998826.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MAL03_RS03130 (MAL03_03130)	748868..749224	-	357	WP_141598270.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MAL03_RS03135 (MAL03_03135)	749221..749481	-	261	WP_002178286.1	type II toxin-antitoxin system HicA family toxin	Toxin
MAL03_RS03140 (MAL03_03140)	749747..750136	-	390	WP_141598271.1	type II toxin-antitoxin system MqsA family antitoxin	-
MAL03_RS03145 (MAL03_03145)	750133..750444	-	312	WP_141598272.1	type II toxin-antitoxin system MqsR family toxin	-
MAL03_RS03150 (MAL03_03150)	750738..751742	-	1005	WP_141598273.1	DUF2971 domain-containing protein	-
MAL03_RS03155 (MAL03_03155)	752217..752630	-	414	WP_141598587.1	helix-turn-helix transcriptional regulator	-
MAL03_RS03160 (MAL03_03160)	752789..753046	+	258	Protein_631	helix-turn-helix transcriptional regulator	-
MAL03_RS03165 (MAL03_03165)	753318..754154	+	837	WP_141598588.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10032.49 Da
• Isoelectric Point: 10.2818

>T235289 WP_002178286.1 NZ_CP091957:c749481-749221 [Leptospira noguchii]
VSKSEKLIQRFLGRPKDFSYDELRKLFSGFGYIEDNSGKSSGSRVAWIHSETKHIIRLHKPHPKNILKSYQINQILDELK
SEGYIK

Nucleotide

• Length: 261 bp

>T235289 NZ_LR134236:996270-996605 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.59 Da
• Isoelectric Point: 7.2781

>AT235289 WP_141598270.1 NZ_CP091957:c749224-748868 [Leptospira noguchii]
MKDIIEYNGFLGSVHFDADDEVFFGKIEGIEDLVSFEGESVKQLKKAFQESVDDYLILCKKAKKSPEKSFKGSFNVRIPT
ELHRRVYRKSLREGISLNQLVQRALEKEITELKTPHFG

Nucleotide

• Length: 357 bp

>AT235289 NZ_LR134236:996022-996270 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: T0FDD9