Detailed information of TA system TA235287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 79638..80209
• Replicon: plasmid p1_LIP1605021
• Accession: NZ_CP091955
• Organism: Leptospira noguchii strain IP1605021

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL09_RS21685
• Protein ID: WP_000240418.1
• Coordinates: 79868..80209 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL09_RS21680
• Protein ID: WP_000443825.1
• Coordinates: 79638..79874 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL09_RS21655 (MAL09_21655)	75455..75799	+	345	WP_036026199.1	hypothetical protein	-
MAL09_RS21660 (MAL09_21660)	75786..76328	+	543	WP_061245396.1	hypothetical protein	-
MAL09_RS21665 (MAL09_21665)	76356..76505	+	150	WP_155717419.1	hypothetical protein	-
MAL09_RS21670 (MAL09_21670)	77274..78128	+	855	WP_141599445.1	hypothetical protein	-
MAL09_RS21675 (MAL09_21675)	78375..79328	+	954	WP_141599443.1	DUF2971 domain-containing protein	-
MAL09_RS21680 (MAL09_21680)	79638..79874	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL09_RS21685 (MAL09_21685)	79868..80209	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL09_RS21690 (MAL09_21690)	80267..80653	-	387	Protein_67	hypothetical protein	-
MAL09_RS21695 (MAL09_21695)	80619..80927	-	309	WP_002148401.1	hypothetical protein	-
MAL09_RS21700 (MAL09_21700)	81594..81839	+	246	WP_004420603.1	ribbon-helix-helix domain-containing protein	-
MAL09_RS21705 (MAL09_21705)	81839..82243	+	405	WP_004420606.1	putative toxin-antitoxin system toxin component, PIN family	-
MAL09_RS22735	82283..82414	-	132	WP_279575927.1	hypothetical protein	-
MAL09_RS21710 (MAL09_21710)	82506..83516	-	1011	Protein_72	IS5 family transposase	-
MAL09_RS21715 (MAL09_21715)	83648..83929	-	282	Protein_73	transposase	-
MAL09_RS21720 (MAL09_21720)	84037..84681	-	645	WP_243808020.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..169819	169819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235287 WP_000240418.1 NZ_CP091955:79868-80209 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235287 NZ_LR134236:716488-716784 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235287 WP_000443825.1 NZ_CP091955:79638-79874 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235287 NZ_LR134236:716786-717181 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33