Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3053550..3054121 Replicon chromosome
Accession NZ_CP091953
Organism Leptospira noguchii strain IP1605021

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag MAL09_RS13585 Protein ID WP_000240418.1
Coordinates 3053780..3054121 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag MAL09_RS13580 Protein ID WP_000443825.1
Coordinates 3053550..3053786 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAL09_RS13550 (MAL09_13550) 3049222..3050077 + 856 Protein_2731 IS982 family transposase -
MAL09_RS13555 (MAL09_13555) 3050132..3051279 + 1148 WP_243807739.1 IS3 family transposase -
MAL09_RS13560 (MAL09_13560) 3051534..3052208 + 675 WP_141599490.1 hypothetical protein -
MAL09_RS13565 (MAL09_13565) 3052461..3052658 + 198 WP_181757029.1 hypothetical protein -
MAL09_RS13570 (MAL09_13570) 3052720..3053112 - 393 WP_004419230.1 transcriptional regulator -
MAL09_RS13575 (MAL09_13575) 3053113..3053451 - 339 WP_243807197.1 type II toxin-antitoxin system HigB family toxin -
MAL09_RS13580 (MAL09_13580) 3053550..3053786 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MAL09_RS13585 (MAL09_13585) 3053780..3054121 + 342 WP_000240418.1 endoribonuclease MazF Toxin
MAL09_RS13590 (MAL09_13590) 3054468..3054733 + 266 Protein_2739 IS5/IS1182 family transposase -
MAL09_RS13595 (MAL09_13595) 3054865..3055137 + 273 Protein_2740 IS5/IS1182 family transposase -
MAL09_RS13600 (MAL09_13600) 3055667..3056229 + 563 Protein_2741 IS5/IS1182 family transposase -
MAL09_RS13605 (MAL09_13605) 3056309..3056494 + 186 WP_141599671.1 hypothetical protein -
MAL09_RS13610 (MAL09_13610) 3056665..3057534 - 870 WP_243807198.1 Rpn family recombination-promoting nuclease/putative transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T235286 WP_000240418.1 NZ_CP091953:3053780-3054121 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T235286 NZ_LR134236:653820-654134 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT235286 WP_000443825.1 NZ_CP091953:3053550-3053786 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT235286 NZ_LR134236:654131-654547 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References