Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 51580..52151 Replicon plasmid p2_LIP1611024
Accession NZ_CP091950
Organism Leptospira noguchii strain IP1611024

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag MAL00_RS19815 Protein ID WP_000240418.1
Coordinates 51810..52151 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag MAL00_RS19810 Protein ID WP_000443825.1
Coordinates 51580..51816 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAL00_RS19780 (MAL00_19780) 47227..47571 + 345 WP_036026199.1 hypothetical protein -
MAL00_RS19785 (MAL00_19785) 47558..48100 + 543 WP_061251579.1 hypothetical protein -
MAL00_RS19790 (MAL00_19790) 48128..48277 + 150 WP_155717419.1 hypothetical protein -
MAL00_RS19795 (MAL00_19795) 48270..48584 + 315 WP_002149298.1 hypothetical protein -
MAL00_RS19800 (MAL00_19800) 49019..50308 + 1290 WP_061251578.1 hypothetical protein -
MAL00_RS19805 (MAL00_19805) 50480..51277 + 798 WP_061251577.1 TIGR02391 family protein -
MAL00_RS19810 (MAL00_19810) 51580..51816 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MAL00_RS19815 (MAL00_19815) 51810..52151 + 342 WP_000240418.1 endoribonuclease MazF Toxin
MAL00_RS19820 (MAL00_19820) 52221..52595 - 375 WP_004455818.1 hypothetical protein -
MAL00_RS19825 (MAL00_19825) 52561..52869 - 309 WP_002148401.1 hypothetical protein -
MAL00_RS19830 (MAL00_19830) 52930..53094 - 165 WP_004420657.1 hypothetical protein -
MAL00_RS19835 (MAL00_19835) 53536..53781 + 246 WP_004420603.1 ribbon-helix-helix domain-containing protein -
MAL00_RS19840 (MAL00_19840) 53781..54185 + 405 WP_004420606.1 putative toxin-antitoxin system toxin component, PIN family -
MAL00_RS20540 54225..54356 - 132 WP_279575927.1 hypothetical protein -
MAL00_RS19845 (MAL00_19845) 54448..55568 - 1121 Protein_49 hypothetical protein -
MAL00_RS19850 (MAL00_19850) 55709..55980 - 272 Protein_50 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74082 74082
- flank IS/Tn - - 55732..55980 248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T235282 WP_000240418.1 NZ_CP091950:51810-52151 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T235282 NZ_LR134236:183737-183844 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT235282 WP_000443825.1 NZ_CP091950:51580-51816 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT235282 NZ_LR134236:c183679-183623 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References