Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 738331..738907 Replicon chromosome
Accession NZ_CP091947
Organism Leptospira noguchii strain IP1611024

Toxin (Protein)


Gene name chpK Uniprot ID -
Locus tag MAL00_RS03030 Protein ID WP_036034136.1
Coordinates 738331..738672 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID M6U786
Locus tag MAL00_RS03035 Protein ID WP_004425715.1
Coordinates 738659..738907 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAL00_RS03020 (MAL00_03020) 733534..733812 - 279 WP_002155541.1 GNAT family N-acetyltransferase -
MAL00_RS03025 (MAL00_03025) 734934..736808 + 1875 WP_004441979.1 ATP-dependent DNA helicase RecQ -
MAL00_RS03030 (MAL00_03030) 738331..738672 - 342 WP_036034136.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MAL00_RS03035 (MAL00_03035) 738659..738907 - 249 WP_004425715.1 ribbon-helix-helix domain-containing protein Antitoxin
MAL00_RS03040 (MAL00_03040) 739283..739429 - 147 WP_004441990.1 hypothetical protein -
MAL00_RS03045 (MAL00_03045) 739508..740316 + 809 WP_243829760.1 IS5 family transposase -
MAL00_RS03050 (MAL00_03050) 740839..741867 - 1029 WP_004437796.1 DUF4238 domain-containing protein -
MAL00_RS03055 (MAL00_03055) 741962..742297 - 336 WP_162267168.1 XRE family transcriptional regulator -
MAL00_RS03060 (MAL00_03060) 742504..742761 + 258 Protein_605 helix-turn-helix transcriptional regulator -
MAL00_RS03065 (MAL00_03065) 742924..743397 - 474 WP_004437841.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12383.36 Da        Isoelectric Point: 10.3684

>T235281 WP_036034136.1 NZ_CP091947:c738672-738331 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSKPGFKRPVLIIQDDSFNQSNISTVVSIVITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Download         Length: 342 bp

>T235281 NZ_LR134236:183254-183361 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9337.83 Da        Isoelectric Point: 10.3019

>AT235281 WP_004425715.1 NZ_CP091947:c738907-738659 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSNKSKELKSNIVDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT235281 NZ_LR134236:c183197-183140 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB M6U786

References