Detailed information of TA system TA235280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 40374..40945
• Replicon: plasmid p2_LIP1703027
• Accession: NZ_CP091946
• Organism: Leptospira noguchii strain IP1703027

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL01_RS19495
• Protein ID: WP_000240418.1
• Coordinates: 40604..40945 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL01_RS19490
• Protein ID: WP_000443825.1
• Coordinates: 40374..40610 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL01_RS19460 (MAL01_19460)	36022..36366	+	345	WP_036026199.1	hypothetical protein	-
MAL01_RS19465 (MAL01_19465)	36353..36895	+	543	WP_061251579.1	hypothetical protein	-
MAL01_RS19470 (MAL01_19470)	36923..37072	+	150	WP_155717419.1	hypothetical protein	-
MAL01_RS19475 (MAL01_19475)	37065..37379	+	315	WP_002149298.1	hypothetical protein	-
MAL01_RS19480 (MAL01_19480)	37814..39103	+	1290	WP_061251578.1	hypothetical protein	-
MAL01_RS19485 (MAL01_19485)	39275..40072	+	798	WP_061251577.1	TIGR02391 family protein	-
MAL01_RS19490 (MAL01_19490)	40374..40610	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL01_RS19495 (MAL01_19495)	40604..40945	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL01_RS19500 (MAL01_19500)	41015..41384	-	370	Protein_28	hypothetical protein	-
MAL01_RS19505 (MAL01_19505)	41381..41659	-	279	WP_243824196.1	hypothetical protein	-
MAL01_RS19510 (MAL01_19510)	42328..42573	+	246	WP_004420603.1	ribbon-helix-helix domain-containing protein	-
MAL01_RS19515 (MAL01_19515)	42573..42977	+	405	WP_004420606.1	putative toxin-antitoxin system toxin component, PIN family	-
MAL01_RS19520 (MAL01_19520)	43240..44360	-	1121	Protein_32	hypothetical protein	-
MAL01_RS19525 (MAL01_19525)	44501..44772	-	272	Protein_33	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61325	61325
	-	flank	IS/Tn	-	-	44524..44772	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235280 WP_000240418.1 NZ_CP091946:40604-40945 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235280 NZ_CP091946:40604-40945 [Leptospira noguchii]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATTGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAAACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAACTATTAATATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235280 WP_000443825.1 NZ_CP091946:40374-40610 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235280 NZ_CP091946:40374-40610 [Leptospira noguchii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33