Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 735489..736065 Replicon chromosome
Accession NZ_CP091943
Organism Leptospira noguchii strain IP1703027

Toxin (Protein)


Gene name chpK Uniprot ID -
Locus tag MAL01_RS02970 Protein ID WP_002155521.1
Coordinates 735489..735830 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID M6U786
Locus tag MAL01_RS02975 Protein ID WP_004425715.1
Coordinates 735817..736065 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAL01_RS02960 (MAL01_02960) 730808..731086 - 279 WP_002155541.1 GNAT family N-acetyltransferase -
MAL01_RS02965 (MAL01_02965) 731967..733841 + 1875 WP_004425742.1 ATP-dependent DNA helicase RecQ -
MAL01_RS19615 734913..735044 + 132 WP_279575168.1 hypothetical protein -
MAL01_RS02970 (MAL01_02970) 735489..735830 - 342 WP_002155521.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MAL01_RS02975 (MAL01_02975) 735817..736065 - 249 WP_004425715.1 ribbon-helix-helix domain-containing protein Antitoxin
MAL01_RS02980 (MAL01_02980) 736431..737099 - 669 Protein_591 DUF4238 domain-containing protein -
MAL01_RS02985 (MAL01_02985) 737124..737597 - 474 WP_243823874.1 IS5 family transposase -
MAL01_RS02990 (MAL01_02990) 737588..737902 - 315 WP_152926911.1 transposase -
MAL01_RS02995 (MAL01_02995) 737922..738134 - 213 WP_053523145.1 hypothetical protein -
MAL01_RS03000 (MAL01_03000) 738778..739623 - 846 WP_243820437.1 hypothetical protein -
MAL01_RS03005 (MAL01_03005) 739625..740905 - 1281 WP_004437627.1 phosphoribosylamine--glycine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12329.22 Da        Isoelectric Point: 9.8437

>T235279 WP_002155521.1 NZ_CP091943:c735830-735489 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSSISTVVSIAITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Download         Length: 342 bp

>T235279 NZ_LR134235:4584759-4584861 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9337.83 Da        Isoelectric Point: 10.3019

>AT235279 WP_004425715.1 NZ_CP091943:c736065-735817 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSNKSKELKSNIVDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT235279 NZ_LR134235:c4584867-4584723 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB M6U786

References