Detailed information of TA system TA235277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 728432..729008
• Replicon: chromosome
• Accession: NZ_CP091940
• Organism: Leptospira noguchii strain IP1705032

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: MAL05_RS02855
• Protein ID: WP_002155521.1
• Coordinates: 728432..728773 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: M6U786
• Locus tag: MAL05_RS02860
• Protein ID: WP_004425715.1
• Coordinates: 728760..729008 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL05_RS02845 (MAL05_02845)	723763..724041	-	279	WP_002155541.1	GNAT family N-acetyltransferase	-
MAL05_RS02850 (MAL05_02850)	724918..726792	+	1875	WP_004425742.1	ATP-dependent DNA helicase RecQ	-
MAL05_RS19215	727864..727995	+	132	WP_279575168.1	hypothetical protein	-
MAL05_RS02855 (MAL05_02855)	728432..728773	-	342	WP_002155521.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAL05_RS02860 (MAL05_02860)	728760..729008	-	249	WP_004425715.1	ribbon-helix-helix domain-containing protein	Antitoxin
MAL05_RS02865 (MAL05_02865)	729276..729548	-	273	WP_004425613.1	BrnA antitoxin family protein	-
MAL05_RS02870 (MAL05_02870)	729535..729834	-	300	WP_004425722.1	BrnT family toxin	-
MAL05_RS02875 (MAL05_02875)	730181..730504	-	324	WP_243825533.1	hypothetical protein	-
MAL05_RS02880 (MAL05_02880)	730786..731367	-	582	WP_232436043.1	hypothetical protein	-
MAL05_RS02885 (MAL05_02885)	732477..733322	-	846	WP_002155522.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12329.22 Da
• Isoelectric Point: 9.8437

>T235277 WP_002155521.1 NZ_CP091940:c728773-728432 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSSISTVVSIAITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Nucleotide

• Length: 342 bp

>T235277 NZ_LR134235:2993157-2993504 [Klebsiella variicola]
ATGTGGGATGCAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9337.83 Da
• Isoelectric Point: 10.3019

>AT235277 WP_004425715.1 NZ_CP091940:c729008-728760 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSNKSKELKSNIVDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT235277 NZ_LR134235:2993504-2993803 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: M6U786