Detailed information of TA system TA235276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 775967..776543
• Replicon: chromosome
• Accession: NZ_CP091936
• Organism: Leptospira noguchii strain IP1709037

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: MAL08_RS03355
• Protein ID: WP_002155521.1
• Coordinates: 775967..776308 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: A0A829BWD5
• Locus tag: MAL08_RS03360
• Protein ID: WP_004450848.1
• Coordinates: 776295..776543 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL08_RS03345 (MAL08_03345)	771510..771659	-	150	WP_243818347.1	hypothetical protein	-
MAL08_RS03350 (MAL08_03350)	772235..774109	+	1875	WP_004425742.1	ATP-dependent DNA helicase RecQ	-
MAL08_RS03355 (MAL08_03355)	775967..776308	-	342	WP_002155521.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAL08_RS03360 (MAL08_03360)	776295..776543	-	249	WP_004450848.1	ribbon-helix-helix domain-containing protein	Antitoxin
MAL08_RS03365 (MAL08_03365)	776794..777159	-	366	WP_155243119.1	XRE family transcriptional regulator	-
MAL08_RS03370 (MAL08_03370)	777363..777620	+	258	Protein_677	helix-turn-helix transcriptional regulator	-
MAL08_RS03375 (MAL08_03375)	778122..778436	-	315	WP_004432609.1	hypothetical protein	-
MAL08_RS03380 (MAL08_03380)	778481..779428	-	948	WP_243818348.1	phage Gp37/Gp68 family protein	-
MAL08_RS03385 (MAL08_03385)	779496..779681	-	186	Protein_680	hypothetical protein	-
MAL08_RS03390 (MAL08_03390)	779699..780420	-	722	Protein_681	hypothetical protein	-
MAL08_RS03395 (MAL08_03395)	780420..781307	-	888	WP_243818349.1	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	766065..814879	48814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12329.22 Da
• Isoelectric Point: 9.8437

>T235276 WP_002155521.1 NZ_CP091936:c776308-775967 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSSISTVVSIAITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Nucleotide

• Length: 342 bp

>T235276 NZ_LR134235:2966869-2967237 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9308.83 Da
• Isoelectric Point: 10.3019

>AT235276 WP_004450848.1 NZ_CP091936:c776543-776295 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSIKSKELKSNIADLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT235276 NZ_LR134235:2966651-2966872 [Klebsiella variicola]
ATGAAAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829BWD5