Detailed information of TA system TA235275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 40260..40831
• Replicon: plasmid p2_LIP1712055
• Accession: NZ_CP091931
• Organism: Leptospira noguchii strain IP1712055

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL02_RS19090
• Protein ID: WP_000240418.1
• Coordinates: 40490..40831 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL02_RS19085
• Protein ID: WP_000443825.1
• Coordinates: 40260..40496 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL02_RS19055 (MAL02_19055)	35909..36253	+	345	WP_036026199.1	hypothetical protein	-
MAL02_RS19060 (MAL02_19060)	36240..36781	+	542	Protein_20	hypothetical protein	-
MAL02_RS19065 (MAL02_19065)	36809..36958	+	150	WP_155717419.1	hypothetical protein	-
MAL02_RS19070 (MAL02_19070)	36951..37265	+	315	WP_002149298.1	hypothetical protein	-
MAL02_RS19075 (MAL02_19075)	37700..38989	+	1290	WP_061251578.1	hypothetical protein	-
MAL02_RS19080 (MAL02_19080)	39161..39958	+	798	WP_061251577.1	TIGR02391 family protein	-
MAL02_RS19085 (MAL02_19085)	40260..40496	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL02_RS19090 (MAL02_19090)	40490..40831	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL02_RS19095 (MAL02_19095)	40901..41270	-	370	Protein_27	hypothetical protein	-
MAL02_RS19100 (MAL02_19100)	41267..41545	-	279	WP_243824196.1	hypothetical protein	-
MAL02_RS19105 (MAL02_19105)	41608..41772	-	165	WP_004420657.1	hypothetical protein	-
MAL02_RS19110 (MAL02_19110)	42214..42459	+	246	WP_004420603.1	ribbon-helix-helix domain-containing protein	-
MAL02_RS19115 (MAL02_19115)	42459..42863	+	405	WP_004420606.1	putative toxin-antitoxin system toxin component, PIN family	-
MAL02_RS19120 (MAL02_19120)	43126..44246	-	1121	Protein_32	hypothetical protein	-
MAL02_RS19125 (MAL02_19125)	44387..44658	-	272	Protein_33	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	44410..44658	248
	-	inside	Non-Mobilizable plasmid	-	-	1..61322	61322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235275 WP_000240418.1 NZ_CP091931:40490-40831 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235275 NZ_LR134235:c2567497-2567114 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235275 WP_000443825.1 NZ_CP091931:40260-40496 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235275 NZ_LR134235:c2567739-2567497 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33